Genetic diversity analysis of artocarpus heterophyllus Lam. using scot markers for antitoxidant and anti inflamminatory propeties
Loading...
Files
Date
2020
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Plant Biotechnology, College of Agriculture, Vellayani
Abstract
The research work entitled “Genetic diversity analysis of Artocarpus heterophyllus
Lam. using SCoT markers for antioxidant and anti-inflammatory properties” was carried out at
the Biotechnology and Bioinformatics Division and Ethnomedicine and Ethnopharmacology
division of Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI),
Palode during the period 2019 – 2020.
Artocarpus heterophyllus Lam., the official fruit of Kerala, is well known for its dietary
uses from ancient times. Cultivar and wild accession comparative studies are not yet reported
based on the genetic variation and phytoconstituents. Present study aimed to analyze the genetic
variation and constituents of phytochemicals for anti-inflammatory property using Start Codon
Targeted (SCoT) marker in both cultivar and wild accessions.
The variation in genetic diversity of both cultivar and wild accessions were
analyzed using 10 SCoT markers. NTSYS and POPGENE were employed for the construction
of dendrogram and estimation of genetic variation. MG 91 cultivar showed the highest variation
with similarity coefficient 0.44 to MG NL1. The total phenolics and flavonoids of the flakes and
seeds were estimated using phytochemical analysis.
High phenolic content was observed in seeds whereas flakes showed high
flavonoids. Antioxidant potential was determined using DPPH radical scavenging activity. MG
92 cultivar flakes which showed the maximum radical scavenging activity with IC50 of 210.58
was used for the in vivo and in vitro anti-inflammatory studies. In anti-inflammatory studies, the
percentage of inhibition showed a dose dependent relation, as the concentration of sample
increased the activity of protection also increased.
The variation in the phytoconstituents of jackfruit differs due to genetic as well as
environmental conditions and also depends on the maturity patterns. It has been noted that
phytoconstituents in the seed and flake samples showed great variation as in the PCR amplified
products from corresponding leaf samples of the jackfruit accessions. Wild accessions studies
are comparatively less but are a potent source for many phytoconstituents with medicinal
properties that need to be investigated.
Description
MSc
Keywords
Citation
175047