POLYMORPHISM STUDY ON MILK PRODUCTION TRAITS IN GOATS USING DOUBLE DIGEST RESTRICTION ASSOCIATED DNA SEQUENCE

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dc.contributor.advisorRadhika G.
dc.contributor.authorK.MATHIVATHANI
dc.date.accessioned2020-09-23T05:18:17Z
dc.date.available2020-09-23T05:18:17Z
dc.date.issued2019
dc.description.abstractNative goat breeds of Kerala; namely Attappady Black and Malabari, apparently varied in milk production capacity. Milk production data was collected from 100 each of Malabari and Attappady Black goats and the data analysed. Parity (p≤0.01), breed (p≤0.05) and herd (p≤0.01) showed significant influence on milk production. Fat per cent showed significant (p≤0.05) breed differences during early, middle and late lactation. Double digest Restriction Associated DNA Sequencing (ddRADseq) is a modern approach for identification of Single Nucleotide Polymorphisms (SNP) across populations. The ddRADseq technique was performed in three pooled samples (ten each) of high and low milk producing Malabari and Attappady Black goats. The SNPs discovered were analysed using bioinformatics tool Venny 3. A total of 4141 and 2192 variants were observed in Malabari and Attappady Black goats respectively. A panel of 42 candidate genes affecting milk production were screened and 26 SNPs were discovered in 22 genes comprising of 19 intron, five downstream and two upstream variants. From a set of 11 milk production related genes, 11 SNPs were identified based on the difference in allelic distribution of SNPs between the two groups. NRXN3, LPL, GLI2 were selected for validation studies. Intronic variant c.301+155748C>T of NRXN3 was validated using High Resolution Melting analysis and showed significant (p≤0.01) association, with mean daily milk yield (mL) of 558.17±4.58, 462.21 ±16.53 and 416.62 ±11.13 for CC, CT and TT genotypes, respectively. In Attappady Black goats, CC genotype was absent as indicated by ddRADseq results. Intronic variant c.243+1082C>T LPL, was validated using Polymerase Chain Reaction-Single Strand Conformation Polymorphism and further sequencing. It had significant (p≤0.05) association with mean daily milk yield (mL) of 437.12±12.85, 494.72±11.60 and 467.63 ± 19.86 for CC, CT and TT genotypes respectively. The GLI2 variant c.64T>G revealed monomorphic pattern in PCR-RFLP. Expression study of BTN1A1 revealed significantly (p≤0.05) higher expression (6.74 fold) in Malabari than Attappady Black. Current study emphasized the relevance of ddRADseq in identifying novel SNPs in candidate and unexplored genes influencing economic traits.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151809
dc.keywordsPOLYMORPHISM STUDY MILK PRODUCTION TRAITS GOATS USING DOUBLE DIGEST RESTRICTION ASSOCIATED DNA SEQUENCEen_US
dc.language.isoEnglishen_US
dc.pages98en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemPOLYMORPHISM STUDY ON MILK PRODUCTION TRAITS IN GOATS USING DOUBLE DIGEST RESTRICTION ASSOCIATED DNA SEQUENCEen_US
dc.subAnimal Genetics and Breedingen_US
dc.themePOLYMORPHISM STUDY ON MILK PRODUCTION TRAITS IN GOATS USING DOUBLE DIGEST RESTRICTION ASSOCIATED DNA SEQUENCEen_US
dc.these.typeM.V.Sc.en_US
dc.titlePOLYMORPHISM STUDY ON MILK PRODUCTION TRAITS IN GOATS USING DOUBLE DIGEST RESTRICTION ASSOCIATED DNA SEQUENCEen_US
dc.typeThesisen_US
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