Contribution of antioxidative defense mechanism and glyoxalase system towards yellow mosaic disease resistance in black gram (Vigna mungo (L.) Hepper)
Loading...
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present investigation was carried out to study the contribution of antioxidative defense
mechanism and glyoxalase system towards yellow mosaic disease (YMD) resistance in three
black gram cultivars KUG 253 (highly susceptible to YMD), MASH114 (resistant) and Cross
(KUG 253 × MASH114) were sown during kharif and spring season. In the kharif season
yellow mosaic virus disease (YMD) infestation was observed in the leaves of KUG 253
cultivar while no signs of infection were visible in the other two cultivars. The severity of
infection increased with the passage of time in the KUG 253 cultivar at 40 DAS during the
kharif season whereas during spring season the YMD infestation was absent in all the three
cultivars of black gram. Due to their differential response against YMD in the kharif season,
various physiological and biochemical parameters were studied. It was found that
antioxidative system is interlinked with the glyoxalase system to provide resistance against
this disease. Higher activities of SOD, CAT and GR along with lower accumulation of MG,
increase in reduced glutathione and free radical scavenging activity in MASH114 at 40DAS
might be responsible for providing resistance against YMD. Enhanced activities of
phenylpropanoid pathway and proline biosynthesis enzymes might be responsible for
impeding the spread of the disease at 40DAS during the kharif season. It was found that Cross
cultivar’s response against YMD was better than KUG253 but not as good as MASH114
cultivar. In the spring season, enzymes of the glyoxalase cycle and MG levels remained low
in all the cultivars due to the absence of the disease and variations in specific activities of the
antioxidative enzymes were observed due to the development changes. High activities of
APX and GR which is responsible for maintaining glutathione pool may have contributed
towards the better growth of the cultivars in the spring season.
Description
Keywords
null