ASSOCIATION OF BUTYROPHILIN GENE POLYMORPHISMS WITH MILK YIELD AND FAT PER CENT IN CROSSBRED CATTLE OF KERALA
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Date
2021-09-29
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Kerala Veterinary and Animal Sciences University,Pookode Wayanad
Abstract
Genetic variability of butyrophilin (BTN1A1) gene among 151 crossbred cattle
of Kerala was investigated and a total of 17 single nucleotide polymorphisms (SNPs)
were detected while 13 being novel. Among the SNPs detected three polymorphisms
G21A, A465G and G506A were selected for detailed analysis. The study population
was genotyped using high resolution melt (HRM) curve analysis and two genotypes
GG (0.86) and GA (0.14) were revealed for G21A polymorphism, while KK (0.25) and
KL (0.75) for A465G transition and MM (0.25) and MN (0.75) with respect to G506A
polymorphism. Chi-square analysis revealed the presence of population in H-W
equilibrium only with respect to SNP G21A. General linear model-univariate analysis
was carried out to study the association of SNPs with milk production traits by
considering herd, season of calving and parity as non-genetic factors and trait as a
dependent variable. Association analysis of G21A revealed significantly higher 305-
day milk yield (GG: 2720.74 ± 122.92 kg; GA: 3250.20 ± 183.24 kg), fat yield (GG:
106.55 ± 4.32 kg; GA: 126.30 ± 13.35 kg) and SNF yield (GG: 211.52 ± 9.20 kg; GA:
246.90 ± 13.70 kg) for GA genotype (P≤0.05) and SNF per cent (GG: 7.80 ± 0.04 %;
GA: 7.65 ± 0.07 %) for GG genotype. Similarly, MN genotype of G506A had shown
significantly higher (P≤0.05) 305-day milk yield (MM: 2358.06 ± 141.46 kg; MN:
3097.51 ± 121.92 kg), fat yield (MM: 94.14 ± 5.00 kg; MN: 119.95 ± 4.31 kg), SNF
yield (MM: 183.14 ± 10.50 kg; MN: 239.08 ± 9.03 kg) and MM genotype for fat per
cent (MM: 4.10 ± 0.08 %; MN: 3.90 ± 0.70 %). However, KK genotype of A465G had
shown a significantly higher (P≤0.01) fat per cent (KK: 4.14 ± 0.08 %; KL: 3.91 ± 0.07
%) and SNF per cent (KK: 7.86 ± 0.06 %; KL: 7.73 ± 0.05 %). The current study
recommends alleles G, K and M as favourable with respect to SNPs G21A, A465G and
G506A, respectively for breeding programmes in crossbred cattle of Kerala.
Description
Submitted in partial fulfillment of the requirement for the degree of
Master of veterinary Science of Animal Genetics and Breeding