MOLECULAR CHARACTERIZATION OF RESTORERS, MAINTAINERS AND CMS LINES OF WILD ABORTIVE CYTOPLASM FOR TAGGING FERTILITY RESTORER GENES IN RICE (Oryza sativa L.)
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Date
2010
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Publisher
Anand Agricultural University, Anand
Abstract
Rice (Oryza sativa L., 2n = 24) is the most important food crop in the
world. Commercial exploitation of heterosis is essential for enhancing
productivity of rice. The use of cytoplasmic male sterility (CMS) and fertility
restoration system greatly facilitates large scale production of hybrid seed.
The wild abortive (WA) cytoplasm is most widely used for hybrid seed
production in rice. Identification of elite restorers and maintainers is needed
for making more promising hybrid combinations. The present investigation
was undertaken to study genetic variability and polymorphism among
restorers, maintainers and CMS lines in rice with WA cytoplasm through
RAPD and SSR molecular marker systems and validate SSR markers
previously reported to be linked to fertility restorer (Rf) gene(s) for WA-CMS
lines of rice through bulked line analysis (BLA). Sufficient amount of quality
genomic DNA was extracted from rice leaves at seedling stage by Cetyl
trimethyl ammonium bromide (CTAB) method for PCR.
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Keywords
Agricultural Biotechnology, Study