Evaluation of Momordica spp. for bioactive components and antidiabetic potential

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dc.contributor.advisorSangha, Manjeet Kaur
dc.contributor.authorSingla, Diksha
dc.date.accessioned2023-12-29T15:10:22Z
dc.date.available2023-12-29T15:10:22Z
dc.date.issued2022
dc.description.abstractThe present study evaluated antidiabetic potential of bioactive components from immature fruits (60 genotypes) of Momordica spp. in streptozotocin-induced diabetic Wistar rats. The fruit and its parts; epicarp, mesocarp, endocarp, and seeds were evaluated for biochemicals. PAUBG-407 possessed higher phenolics, fiber components, carotenoids, saponins, macro and microminerals; PAUBG-195 (alkaloids); PAUBG-130 (ascorbic acid and tocopherols); PAUBG-218 (proteins) and Punjab-15 (most bioactives). Whole fruit was rich in many bioactives. Charantin, soluble dietary fiber and momordicin-II were purified from whole fruits of PAUBG-407 while vicine and momordicin-I were purified from whole fruit of PAUBG- 195 and PAUBG-89, respectively and characterized on NMR and FT-IR. Their antidiabetic potential was evaluated in 7-8 week old rats, orally administered bioactives @100mg/kg b.w, daily for 4 weeks. Nine groups were made; normal (), untreated diabetic (II), diabetic + (metformin (I), charantin (IV), SDF (V), vicine (VI), momordicin-I (VII), momordicin-II (V), and diabetic+ all bioactives mixed in equal ratio (IX)). Their effect was assessed weekly and at 4 weeks (some parameters). Fasting and random blood glucose; kidney and Iiver CAT, SOD, GPx, GST, GSH. and TBARS: KFT (urea, creatinine, uric acid, Na', K and Ca); LFT (bilirubin. direct and indirect bilirubin, SGOT, SGPT, ALP, total protein, albumin and globulin): plasma insulin, Hb and HbAl c; serum lipid profile (TC, TG, VLDL, DL and LDL), hepatic enzymes- hexokinase, glucose-6-phosphatase and fructose-6- phosphatase; histological aberrations observed in liver, kidney, pancreas and brain improved SIgnificantly (p<0.0001) in diabetic rats treated with all bioactives but highly with momordicin-II and bjoactive mixture. PAUBG-407 may be used in improvement programmes generating antidiabetic karela. Future plans may envisage in testing antidiabetic potential of f targeted bioactives or use of targeted advanced line in diabetic human subjects.
dc.identifier.citationSingla, Diksha (2022). Evaluation of Momordica spp. for bioactive components and antidiabetic potential (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810205012
dc.keywordsMomordica
dc.keywordsfruits
dc.keywordsbioactives
dc.keywordsrats
dc.keywordsantidiabetic ALKshe
dc.language.isoEnglish
dc.pages169
dc.publisherPunjab Agricultural University
dc.research.problemEvaluation of Momordica spp. for bioactive components and antidiabetic potential
dc.subBiochemistry
dc.themeEvaluation of Momordica spp. for bioactive components and antidiabetic potential
dc.these.typePh.D
dc.titleEvaluation of Momordica spp. for bioactive components and antidiabetic potential
dc.typeThesis
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