COMPARISON OF DIFFERENT MOLECULAR TYPING METHODS FOR CHARACTERISATION OF Riemerella anatipestifer ISOLATES

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dc.contributor.advisorPriya P. M
dc.contributor.authorC. DEVIGASRI
dc.date.accessioned2020-09-23T07:33:47Z
dc.date.available2020-09-23T07:33:47Z
dc.date.issued2019
dc.description.abstractThe pathogen causing septicaemia and infectious serositis in ducks and wide variety of wild birds, Riemerella anatipestifer has been categorised as a member of Flavobacteriaceae by 16S rRNA sequence analysis. At present, 21 serotypes have been characterised using serum agglutination test. So far, the molecular determinants for R. anatipestifer serotyping are not clear; moreover, serotype analysis is time-consuming and labour-intensive. To find a better molecular typing method for R. anatipestifer, serotype-associated genetic variations has to be determined. Hence, the 20 field isolates (maintained as lyophilised culture and also collected from the suspected cases reported to the Department of Veterinary Microbiology) were subjected to different molecular methods like Polymerase chain reaction- Restriction fragment length polymorphism (PCR-RFLP), Repetitive-sequence based PCR (REP- PCR), Internal transcribed spacer (ITS) nucleotide sequencing, Omp A gene sequencing and pulsed field gel electrophoresis (PFGE) to determine the genetic variations. The phylogenetic trees constructed based on the variations of the ITS region grouped the organism into four clusters and the amino acid sequence variation of OmpA region categorised it into two clusters. The Omp A sequence correlated much better with serotyping than ITS. By comparing the pattern based methods, REP-PCR and PCR-RFLP showed similarity with PFGE which distinguished the isolates into three pulsotypes, Although all methods displaying broadly similar discriminatory powers, REP-PCR subtyping proved to be a much easier, cheaper and more rapid method. To conclude many techniques are available but each of them has advantages and limitations which making them useful in some studies and restrictive in others. Hence to group the local isolates with universally categorised isolates, one or more of the different typing methods need to be applied in conjunction with conventional methods.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151832
dc.keywordsCOMPARISON DIFFERENT MOLECULAR TYPING METHODS CHARACTERISATION Riemerella anatipestifer ISOLATESen_US
dc.language.isoEnglishen_US
dc.pages79en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemCOMPARISON OF DIFFERENT MOLECULAR TYPING METHODS FOR CHARACTERISATION OF Riemerella anatipestifer ISOLATESen_US
dc.subVeterinary Microbiologyen_US
dc.themeCOMPARISON OF DIFFERENT MOLECULAR TYPING METHODS FOR CHARACTERISATION OF Riemerella anatipestifer ISOLATESen_US
dc.these.typeM.V.Sc.en_US
dc.titleCOMPARISON OF DIFFERENT MOLECULAR TYPING METHODS FOR CHARACTERISATION OF Riemerella anatipestifer ISOLATESen_US
dc.typeThesisen_US
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