DIETARY SUPPLEMENTATION OF DL-METHIONINE ON GROWTH PERFORMANCE AND EXPRESSION OF MYOGENIC GENES IN LOW HATCH WEIGHT BROILER CHICKS
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Date
2018
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The present study was intended to elucidate the effect of DL- methionine
(110 per cent and 120 per cent of BIS recommendation 2007) supplementation on
growth performance and gene expression pattern of myogenic family viz.,
myostatin (mstn), myogenic factor 5 (myf5), myocyte enhancer factor 2 family of
transcription factor B (mef2b) in breast muscles of low hatch weight broiler chicks.
One hundred and sixty numbers of day-old vencobb-400 broiler chicks which
comprising of low and optimum hatch weight (120 and 40 respectively) were
purchased from the local hatchery. The day-old chicks with optimum hatch weight
(42.0 to 45.0 g) were grouped as G1 and chicks with low hatch weight (35.0-38.0g)
were randomly allocated to groups G2, G3 and G4, with four replicates of ten chicks
each by using completely randomised design (CRD). Experimental ration (R1) was
prepared with BIS recommended DL-methionine (0.50, 0.50 and 0.45 per cent for
broiler pre-starter, starter and finisher respectively). The experimental ration (R2)
was prepared with 110 per cent of BIS (2007) recommended methionine (0.55, 0.55
and 0.50 per cent for broiler pre-starter, starter and finisher respectively). The
experimental ration (R3) was prepared with 120 per cent of BIS (2007)
recommended methionine (0.60, 0.60 and 0.54 per cent for broiler pre- starter,
starter and finisher respectively). The groups G1 and G2 were fed with ration R1.
The groups G3 and G4 were fed with rations R2 and R3 respectively, for a period
of 42 days. The four birds form each group was sacrificed for study of carcass yield,
breast yield along with gene expression studies. Dietary supplementation of DLMethionine had no significant effect on cumulative feed intake, body weight gain
and FCR at the end of experiment but G3 showed better performance when
compared with other groups. Over all five per cent mortality was observed. There
was better carcass yield and breast yield in G3 when compared with other groups,
without any significance difference among groups. The myogenic family mstn gene
expression was downregulated in G2 group and upregulated in G3 and G4, but there
was no significant difference between G1 and G2, whereas G3 and G4 showed
significantly upregulation in gene expression study when compared with G1 group.
myf5 gene was upregulated in G1 and G2 but downregulated in G3 and G4 but there
was no significant difference among groups. The mef2b gene was upregulated in all
groups but there was no significant difference between the gene expression among
groups. The profit per kg live weight was highest for G3 than the other groups.
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