Genetic characterization and therapeutic management of Babesia spp. infecting dogs
| dc.contributor.advisor | Agnihotri ,Divya | |
| dc.contributor.author | Ansu Kumari | |
| dc.date.accessioned | 2024-07-23T04:52:39Z | |
| dc.date.available | 2024-07-23T04:52:39Z | |
| dc.date.issued | 2024-03 | |
| dc.description.abstract | Canine babesiosis is a clinically significant and emerging tick-borne disease caused by intraerythrocytic protozoa of the genus Babesia. The present study aimed to study the molecular detection, sequence and phylogenetic analyses, genetic diversity and haplotype network of Babesia spp. infecting dogs, along with their therapeutic management. During the study period (May 2022 to April 2023), 216 blood samples were collected from babesiosis suspected dogs from three north Indian states (Haryana, Rajasthan and Uttar Pradesh) and one union territory (New Delhi). Most common clinical signs observed in the infected dogs were fever, anaemia, enlarged lymph nodes, inappetence to anorexia, lethargy, tachypnea and tachycardia. The percent positivity for canine babesiosis by microscopy and conventional polymerase chain reaction was 12.5 % (27/216) and 27.78 % (60/216), respectively. Results of PCR assay revealed that B. vogeli was predominant species as it was incriminated in 48 cases (22.22%), while B. gibsoni was confirmed in only 12 dogs (5.56%). Phylogenetic analysis based on the 18S rRNA and cyt b genes of B. vogeli revealed the presence of two genotypes and sub-clades, respectively. Likewise, phylogeny of B. gibsoni based on the B. gibsoni thrombospondin-related adhesive protein (BgTRAP) and COXI genes suggested the existence of three genotypes and a monophyletic clade, respectively. Upon sequence analyses of B. vogeli based on the 18S rRNA gene, a higher level of percent identity (97.6-100 %) was documented within genotypes, as compared to between them (92.5-93.8 %). A relatively low percent identity was noticed within (93.9- 100 %) and between (83.3-88.4 %) B. gibsoni genotypes based on the BgTRAP gene. Furthermore, extensive sequence conservation was documented in the cyt b and COXI genes of B. vogeli and B. gibsoni, respectively. A high haplotype and low nucleotide diversities were recorded in the 18S rRNA gene of B. vogeli, and BgTRAP and COXI genes of B. gibsoni. The location-wise haplotype networks exhibited geographical clustering between haplotypes of B. vogeli and B. gibsoni. Dogs in general were found to be infected with the novel haplotypes of B. vogeli and B. gibsoni. Moreover, the population genetic structure, demographic history and therapeutic management of B. vogeli and B. gibsoni were also reported. Among risk factors, B. vogeli infection exhibited significant association with nondescript dogs (OR= 27.49; P= 0.044) and lymph node enlargement (OR= 3.33; P= 0.009). Similarly, B. gibsoni infection exhibited significant association with dogs aged more than four years (P= 0.000) and males (P= 0.042). Haematological parameters, viz., Hb, TEC, PCV and thrombocyte count, were found to be significantly decreased in both B. vogeli and B. gibsoni affected dogs (P <0.05). Biochemical parameters, viz., ALT, TB, DB, IDB and BUN, were found to be significantly increased in canine babesiosis affected dogs (P <0.05) | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810212385 | |
| dc.language.iso | English | |
| dc.pages | 123 + xiii + I | |
| dc.publisher | LUVAS Hisar | |
| dc.sub | Veterinary Medicine | |
| dc.theme | Genetic characterization and therapeutic management of Babesia spp. infecting dogs | |
| dc.these.type | Ph.D | |
| dc.title | Genetic characterization and therapeutic management of Babesia spp. infecting dogs | |
| dc.type | Thesis |