Protein fractionation and lipoxygenase activity of bran from high yielding rice genotypes

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dc.contributor.advisorSharma, Neerja
dc.contributor.authorPooja
dc.date.accessioned2018-12-26T07:15:29Z
dc.date.available2018-12-26T07:15:29Z
dc.date.issued2018
dc.description.abstractIn the present study, rice bran from 19 genotypes, including ten non-basmati and nine basmati genotypes were characterized on the basis of their proximate and nutritional composition along with antioxidant properties of protein fractions. The constitutional level of lipoxygenase in rice bran was also determined. The content of crude protein, crude fat, crude ash and dietary fibre in their bran fraction varied from 12.56-22.88 %; 16.92-23.63 %; 8.43-14.96 % and 21.62-28.48 %, respectively. The mean protein content of various Osborne fractions viz., albumin, globulin, glutelin and prolamin among genotypes was found to be in the ratio of 41.09: 18.33: 36.16: 4.42. Significant variation was seen in free amino acids (4.58-10.95), cysteine (0.91-2.07 mg g-1 DW ), methionine (16.33-27.59 mg g-1 DW), proline (19.48 μg g-1 DW), lysine (3.52-5.86 %) and tryprophan (0.55-1.55 %) content. Antioxidant properties of rice bran protein fractions in terms of DPPH free radical scavenging activity (%), Ferric reducing ability power (μmol Fe (II) g-1 DW) and metal chelating power assay(%) was found in the order of albumin > globulin > glutelins > prolamin. Superoxide anion radical scavenging activity (%) was found to be highest in albumin fraction, followed by glutelins, globulins and prolamin. Low reducing power (mg/g DW) and radical cation ABTS.+ scavenging activity (mM/g TEAC) was present in rice bran protein fractions. Wide differences in the rice bran lipoxygenase activity (0.082-1.81 units) was observed. This information about the variations in biochemical parameters among rice bran from different genotypes would enable the breeders’ to produce genotypes with improved bran proximate composition and ratio of protein fractions with high antioxidant potential and low lipoxygenase activity for desired end-use.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810086319
dc.keywordsRice, Rice bran, Proximate composition, Amino acids, Proteins, Antioxidant potentialen_US
dc.language.isoenen_US
dc.pages77en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemProtein fractionation and lipoxygenase activity of bran from high yielding rice genotypesen_US
dc.subBiochemistryen_US
dc.subjectnullen_US
dc.themeProtein fractionation and lipoxygenase activity of bran from high yielding rice genotypesen_US
dc.these.typeM.Scen_US
dc.titleProtein fractionation and lipoxygenase activity of bran from high yielding rice genotypesen_US
dc.typeThesisen_US
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