ASPEE College of HorticultureMicropropagation in Ber Cv. Gola
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Date
2011-04
Journal Title
Journal ISSN
Volume Title
Publisher
Fruit Science Department, ASPEE College of Horticulture and Forestry Navsari Agricultural University
Abstract
The present investigation was carried out at the
Department of Biotechnology, ASPEE College of Horticulture and
Forestry, Navsari Agricultural University, Navsari , Gujarat on
"Micropropagation in ber cv. Gola" during the year 2008-2010.
The surface sterilization of explants, treatment involving
HgCI2 (0.1 %) for 3 minutes gave better sterilization of shoot tips and
axillary bud from seedling and axillary bud from mature explants.
Although, contamination was less in seedling explants, establishment
of explants was higher in explants of mature plant. The use of
mercuric chloride alone was more effective in reducing the
contamination per cent as compared to sodium hypochloride.
. .
Maximum per cent establi shment of all type of explants
viz. shoot tips and axillary bud from seedling explants and axillary
bud from mature explants were observed on tre atm ent MS medium
containing 0.4 mg!1 BAP.
I
MS medium supplemented with 2.0 mg/l BAP exhibited
maximum multiplication rate of shoot with higher length of shoots
in axillary bud from mature explants. In serial sub cultures,
subsequently, multiple shoots and length of shoot (cm) were
reported higher in the same treatment up to three sub cultures.
Maximum proliferation of culture was recorded in 3%
sucrose in the medium followed by 4% sucrose.
Media pH had much influence on growth of regenerated
shoots. Among the different pH levels tested, 5.S pH was found to
be the best for maximum number of shoots and length of shoot
followed by 5.7 pH.
Maximum number of shoots, length of shoot and growth
rate was observed at 3000 lux light intensity.
In vitro rooting in regenerated shoots was found the best
with full strength MS medium supplemented with 0.5 mg/l IBA,
which recorded the maximum number of roots/shoot (6.S3) and
length of root (6.73 cm).
In vitro grown plant lets having (6-S em length of shoot)
exhibited better survival of plantlets FYM + soil + sand (I: I: lv/v)
potting mixture. In vitro raised plantlets were acclimatized
successfully under the climatic condition of plantlets covered with
plastic cup in group and kept under net house condition, which
showed better survival of plantlet (76.50%).
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Citation
PRAJAPATI ANKURKUMAR DINESHCHANDRA_46348