Mapping of Karnal Bunt (Tilletia indica Mitra) resistance gene(s) in recombinant inbred lines of bread wheat
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
The current study was conducted to evaluate a set of 972 NBPGR (National Bureau of Plant Genetics and Resources) accessions and three recombinant inbred lines (RILs) derived from Karnal bunt (KB) resistant donors viz., ALDAN, CMH77.308, and H567.71 in the background of susceptible parent WH542 at PAU, Ludhiana during crop seasons from 20192022. Out of the total 972 accessions evaluated for resistance against Tilletia indica; 417 lines were found resistant over the three years. These 417 lines included 302 highly resistant lines with zero percent KB infection and 115 lines having KB infection up to 5.0 percent. The phenotyping of three RIL populations of ALDAN „S‟ IAS 58 x WH 542, 208 lines screened for KB resistance reported 24 lines having complete resistance i.e., 0% KB infection and the rest of the 184 lines exhibited 0.1 to >20 percent infection. In RIL population CMH 77.308 x WH 542, out of 75 lines screened for KB resistance, 15 lines were reported to be completely resistant having 0.00 percent KB infection and the rest 60 lines showed 0.1 to >20 percent infection. Likewise, in the RIL population H567.71/3*PAR x WH542, a total number of 72 lines screened for KB resistance, 12 RILs were reported to be completely resistant i.e., 0% KB infection the rest 60 lines showed 0.1 to >20 percent infection. A further panel of 63 lines from two RIL populations i.e. ALDAN „S‟ IAS 58 x WH542 and H567.71/3*PAR x WH542 were subjected to genotyping using SNP markers. This panel consisted of 55 resistant and 8 highly susceptible lines from the two crosses. As a consequence, the present study has identified three major QTLs: qKB.pau.AW-4A.1, qKB.pau.AW-4A.2, and qKB.pau.AW1A
from ALDAN „S‟ IAS 58 x WH542 RIL populations. Similarly, qKB.pau.HW-1A.1, qKB.pau.HW-1B, and qKB.pau.HW-1A.2 are the notable QTLs detected from the H567.71/3*PAR x WH542 RIL populations during 2019–2020 and 2020–21. The QTLs detected across these environments may be considered more stable and robust as well as may be utilized in the further breeding program for KB resistance.
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Bromand, Ferdaws (2022). Mapping of Karnal Bunt (Tilletia indica Mitra) resistance gene(s) in recombinant inbred lines of bread wheat (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.