EFFECT OF EXOGENOUS PHENOLIC ACIDS ON ANTIOXIDANT RESPONSE IN WHEAT (Triticum aestivum L.)
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Date
2014
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Punjab Agricultural University, Ludhiana
Abstract
In the present study, effect of exogenous salicylic acid (SA), gallic acid (GA) and ferulic acid (FA) on antioxidant response was investigated in wheat seedlings. For this, PBW 621 seeds were pre-treated with varying concentrations of SA, GA and FA and growth data was taken at 6th day after germination (DAG). In comparison to hydroprimed seedlings, application of 10-70 ppm of SA, 20-140 ppm of GA and 50-150 ppm of FA had growth promotory effects in wheat. Although at higher concentrations, SA (200 ppm) and FA (1000 ppm) inhibited seedling growth however GA even at 1000 ppm did not cause inhibitory effects. In comparison to water primed seedlings, 40 and 70 ppm of salicylic acid augmented antioxidant response by upregulating antioxidative enzymes viz. SOD, CAT, POX, APX and GR and increasing proline, ascorbate and total phenolic contents leading to reduced MDA content. Pretreatment with gallic acid (20-140 ppm) led to increase in the GR and APX activities along with the increase in proline, ascorbate and total phenolic contents. Wheat seedlings pre-treated with 100 and 150 ppm of FA showed upregulated CAT and POX activities which resulted in lower H2O2 content. Further, wheat seedlings pre-treated with 100 and 150 ppm of FA showed enhanced ascorbate and total phenolic contents. Therefore, lower concentrations of these phenolic acids i.e. 40 ppm of SA, 80 ppm of GA and 100 ppm of FA could be used to promote the antioxidant response in wheat. Antioxidant response, ferulic acid, gallic acid, salicylic acid, wheat
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