Investigations on cultivation technology of shiitake mushroom (Lentinus edodes (Berk.) Pegler)
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Date
2010
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Publisher
CCSHAU
Abstract
The present studies were carried out to investigate the possibility of growing shiitake
mushroom (Lentinus edodes) under Haryana conditions on locally available substrates. The
physiological studies were done to determine the optimum pH and temperature conditions for
vegetative growth of L. edodes under in vitro conditions. Shiitake required temperature of 25oC and
acidic pH (4-6) for optimum vegetative growth. Among different media, potato dextrose agar
supported maximum growth and Czapek Dox agar supported the least growth. Among different
agro-residue extract media wheat straw extract agar was best for mycelial growth of L. edodes
under in vitro conditions. Paddy straw + sawdust extract agar medium supported the least growth.
The cellulolytic activity of L. edodes was determined to assess its cellulose degrading ability. The
Exo β-1, 4 glucanase activity was 0.018 and 0.019 μmol/ ml/ min and the Endo β-1, 4 glucanase
activity was 0.033 and 0.036 μmol/ ml/ min for strains S1 and S2 respectively. Bajra grains were
most suited for its spawn production.Wheat straw was found to be most suitable substrate for
cultivation of L. edodes under Haryana conditions. The spawn run was earlier on wheat straw. The
low temperature (15oC) induced fruiting in the shiitake mushroom. The wheat straw as substrate
gave higher yield and had more biological efficiency than sawdust used as substrate for cultivation
of shiitake mushroom. The crude protein content of shiitake mushroom was 21.2 per cent.
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Keywords
Ssowing, Nitrogen, Yields, Fruits, Sowing date, Turnips, Planting, Crops, Research methods, Fertilizers