EXPRESSION STUDIES OF DIFFERENTIALLY EXPRESSED GENES DURING LACTATION IN INDIAN BUFFALO BY REAL TIME PCR
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Date
2009
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AAU, Anand
Abstract
With the growing human population of world, there is always demand of food. It is expected that in future demand of food required is going to exceed the supply available. The supply of adequate and nutritious food will going to be very difficult if the population size remains to increase at same pace. Hence, there is always a need to produce good quality food at lowest possible cost and highest possible production. The present study was undertaken with an objective of determining level of expression of some of the ESTs generated after the exogenous administration of recombinant bovine somatotropin (rbST). Real Time Relative Quantification experiment was done to elucidate level of expression of genes in 0 (without treatment of rbST), 48 and 96 (48 and 96 hours after rbST administration) mammary tissue samples collected from Surti buffalo. RNA extraction was done using TRJ- reagent protocol. First strand cDNA synthesis was done using oligodT primer. Real Time PCR was done using gene specific forward and reverse primers designed from available ESTs sequences and SYBR Green Master Mix. Level of expression was deduced using Sequence Detection Software v 1.3.1 (Applied Biosystems). This study showed 13 out of 15 ESTs showing up regulation, 1 was showing down regulation and 1 showing surge in level of expression at 48 hr stage and showed decrease in level of expression at 96 hour stage though it was higher than 0 hr.
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ANIMAL BIOTECHNOLOGY, A STUDY