Genetic mapping of brown plant hopper resistance from Oryza rufipogon (Griff.)
| dc.contributor.advisor | Kumari Neelam | |
| dc.contributor.author | Pavneet Kaur | |
| dc.date.accessioned | 2019-12-14T06:20:03Z | |
| dc.date.available | 2019-12-14T06:20:03Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Brown planthopper (BPH) Nilaparvata lugens (Stal) is one of the most serious insect-pest of rice accounting for the loss up to 52% of annual production. Under high infestation, complete drying and plant death occurs causing “hopper burn”. The primary strategy for controlling BPH is the application of chemical insecticides, which is already proven detrimental not only to the environment, but also to human health. On this account host-plant resistance serves as an important strategy to reduce the damage caused by BPH. Out of 1003 wild species germplasm accessions screened against BPH, O. rufipogon acc. CR100441 has shown consistent resistance reaction (score 1-3) during 3 years of screening under greenhouse conditions. The F1 plant was obtained by making a cross of O. rufipogon acc.CR100441 and PR122. The BC2F2 plants were generated by further back crossing with PR122 and selfing then after. The BC2F2 plants were screened by the standard seedbox technique at seedling stage against BPH biotype 4. The BC2F2 plants were scored phenotypically on a 0-9 scale according to the Standard Evaluation System (IRRI, 1996) as 1-3 resistance, 5 moderate resistance, and 7-9 susceptible plant. From 276 BC2F2 plants, 66 plants were found to be resistant (1-3 score) and 210 plants (5, 7 and 9 score) were susceptible which fitted in 3:1 ratio (susceptible: resistant) indicating the recessive nature of the BPH resistance gene. Genotyping using polymorphic microsatellite markers between PR122 and O.rufipogon acc.CR100441 spanning all the 12 chromosomes of rice was done. The QTL governing BPH resistance was identified on the short arm of chromosome 4 between marker interval RM551 and RM6659. The RM16335 was peak marker, with LOD score of 10.8 and 35.23% contributing towards the phenotypic variation. This information can be used for further fine mapping and cloning of the gene. The pre-breeding BC2F4 lines with BPH resistance can be further utilize in breeding programmes aimed in development of resistant cultivars. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810137701 | |
| dc.keywords | Oryza rufipogon, International Rice Research Institute, Logarithm of the odds, Quantitative trait loci, Rice microsatellite | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 68 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Genetic mapping of brown plant hopper resistance from Oryza rufipogon (Griff.) | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Genetic mapping of brown plant hopper resistance from Oryza rufipogon (Griff.) | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Genetic mapping of brown plant hopper resistance from Oryza rufipogon (Griff.) | en_US |
| dc.type | Thesis | en_US |