INFLUENCE OF DEXAMETHASONE AND EPIDERMAL GROWTH FACTOR SUPPLEMENTATION IN SYNTHETIC OVIDUCTAL FLUID MEDIUM ON IN VITRO BOVINE EMBRYO DEVELOPMENT

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dc.contributor.advisorAbhilash R.S.
dc.contributor.authorRAKSHITHA P
dc.date.accessioned2020-09-17T07:24:53Z
dc.date.available2020-09-17T07:24:53Z
dc.date.issued2019
dc.description.abstractThe study was designed to assess the individual and combined effect of dexamethasone and epidermal growth factor (EGF) on in vitro bovine embryo development. Ovaries of abattoir origin from crossbred cattle of Kerala were subjected to study. All visible follicles of size 2-8 mm were aspirated and culture grade oocytes were subjected to IVM in TCM-199 medium and fertilized in fertTALP medium and embryos were cultured in mSOF. Presumptive zygotes in group I (n= 162) were treated with 0.1µg/ml of dexamethasone; Group II (n= 198) with 5ng/ml of EGF; Group III (n=199) with 0.1µg/ml of dexamethasone and 5 ng/ml of EGF and Group IV (n= 154) kept as control. Embryo developmental competence was assessed every 48 h and morphological evaluation and embryo viability (Hoechst 33342 staining) was performed on day 8. On day 2 significantly higher (p ≤ 0.05) fertilization and cleavage rate was observed in group II (53.04 ± 2.87 and 35.69 ± 1.02 %) and group III (50.34 ± 2.18 and 34.47 ± 1.98 %) compared to group I (36.33 ± 4.15 and 20.05 ± 3.46%) and group IV (33.00 ± 4.38 and 16.29 ± 4.64 %). On day 4 significantly higher 4 and 16 cell stage embryo was obtained in group II and group III than group I and IV. A significantly higher (p≤0.05) 8 cell stage embryo was observed in group III when compared to group I and IV. However there was no significant difference in 8 cell stage embryo was observed between group II and III. On day 6 and 8 morula/ blastocyst yield was significantly higher (p ≤ 0.05) in group II and III (9.06 ± 0.89 and 8.21 ± 2.21) than in Group I and IV (3.63 ± 1.29 and 2.66 ± 1.54). The proportion of good and fair quality embryos were recorded on day 6, group I (75 and 12.5%), II (52.6 and 31.6%), III (77.8 and 5.6%) and IV (28.6 and 57.1%). Higher per cent of morphologically good and fair quality embryos on day 8 were observed in group I (50 and 25%) and III (44.4 and 27.8%) than group II (10.5 and 15.7%) and IV (14.3 and 14.3%). Embryo viability assessed by Hoechst 33342 on day 8 revealed a higher per cent of viable embryos in group I and III (83.33 and 83.33%) when compared to group II and IV (50 and 16.67%). The present study revealed that supplementation of dexamethasone in mSOF improved the quality of embryos. The supplementation of EGF in mSOF improved the yield of embryos and combining dexamethasone and EGF in mSOF resulted in increased number of embryos with better quality.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151425
dc.keywordsINFLUENCE DEXAMETHASONE EPIDERMAL GROWTH FACTOR SUPPLEMENTATION SYNTHETIC OVIDUCTAL FLUID MEDIUM VITRO BOVINE EMBRYO DEVELOPMENTen_US
dc.language.isoEnglishen_US
dc.pages91en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemINFLUENCE OF DEXAMETHASONE AND EPIDERMAL GROWTH FACTOR SUPPLEMENTATION IN SYNTHETIC OVIDUCTAL FLUID MEDIUM ON IN VITRO BOVINE EMBRYO DEVELOPMENTen_US
dc.subAnimal Reproduction, Gynaecology and Obstetricsen_US
dc.themeINFLUENCE OF DEXAMETHASONE AND EPIDERMAL GROWTH FACTOR SUPPLEMENTATION IN SYNTHETIC OVIDUCTAL FLUID MEDIUM ON IN VITRO BOVINE EMBRYO DEVELOPMENTen_US
dc.these.typeM.V.Sc.en_US
dc.titleINFLUENCE OF DEXAMETHASONE AND EPIDERMAL GROWTH FACTOR SUPPLEMENTATION IN SYNTHETIC OVIDUCTAL FLUID MEDIUM ON IN VITRO BOVINE EMBRYO DEVELOPMENTen_US
dc.typeThesisen_US
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INFLUENCE OF DEXAMETHASONE AND EPIDERMAL GROWTH FACTOR SUPPLEMENTATION IN SYNTHETIC OVIDUCTAL FLUID MEDIUM ON IN VITRO BOVINE EMBRYO DEVELOPMENT
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