DIAGNOSIS OF JOHNE'S DISEASE IN CATTLE BY ALLERGIC TEST, PASSIVE HAEM-AGGLUTINATION TEST AND HISTOPATHOLOGICAL EXAMINATION
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Date
1982
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AAU, Anand
Abstract
Johne's disease is an infectious and fatal, chronic, wasting disease of farm animals. The farm economy is greatly affected, bringing financial loss to farmers. Therefore an early detection of the disease in farm animals will benefit the farmers and preventive measures can be taken in time. Various tests are employed to establish the diagnosis of the disease in farm animals however, no single test is specific and sensitive for the detection of the disease and hence timely preventive measures can not be taken. With the above limitations in the diagnosis of Johne's disease in farm animals, the present work was undertaken to study the incidence of Johne's disease in Jersey, Cross-bred and Kankrej animals at Gujarat Agricultural University Farms, Anand employing three major comparative tests during the year 1960 and 1981. During the 1980, 373 animals (Jersey 186, Cross-bred 19 and Kankrej 168; were tested by single intradermal Johnin test and seven animals (1.88 per cent) were positive reactors to this test, while in the year 1981 out of 370 animals (Jersey 168, Cross-bred 79 and Kankrej 123; 18 animals (4.86 per cent; were reactors to the test. Sera samples of these 25 animals were put to passive haemagglutination test and the titre varied from 1:8 to 1:256. These animals were sacrificed and detailed post-mortem examination was carried out. The acid-fast bacilli were detected in ileo-caecal valve and mesentric lymph nodes in all animals, while tissues showed characteristic lesions. Results were discussed and compared along with the merits and demerits of each test and their implications were emphasized. It can be concluded from the results of the present study that the single intradermal test has a definite diagnostic value in the detection of Johne's disease in farm animals.
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VETERINARY MEDICINE, A STUDY