METAGENOMIC BASED MICROBIAL DIVERSITY STUDY OF DAIRY EFFLUENT TREATMENT PLANTS
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Date
2019
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Department of Dairy Microbiology, SMC College of Dairy Science, Anand Agricultural University, Anand
Abstract
The dairy effluent treatment plant (ETP) is a very dynamic biological system, where the resident microbiota functions toward the bioremediation of dairy effluent. Our understanding of ETPs remains limited due to the high complexity of the microbial community and the presence of numerous non-cultivable microbes. To understand the microbiota of dairy ETPs and its dynamics with seasonal variation and physicochemical characteristics of effluents, a metagenomic study using next-generation sequencing was carried out. The physicochemical characterization of dairy effluent from all the sections of ETP with seasonal variation was tested. All the dairy effluents were analyzed for metagenomic 16S rRNA amplicon to know the microbial communities with a list of microbes and relative abundance (Taxonomic diversity). Shotgun sequencing of the effluent samples collected in the summer season from the anaerobic digester and aeration tank of dairy ETPs were carried out to know the microbiota and functional potential of microorganisms.