Integrated nutrient management in forage cowpea with application of liquid microbial inoculants

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dc.contributor.advisorPandove, Gulab
dc.contributor.authorRamya S.
dc.date.accessioned2019-09-12T07:17:18Z
dc.date.available2019-09-12T07:17:18Z
dc.date.issued2019
dc.description.abstractThe present investigation was carried out to evaluate the role of liquid microbial inoculants in integrated nutrient management of forage cowpea (Vigna unguiculata). For the formulation of liquid microbial inoculants, Bacterial isolate 1 (CP-AK-SPS-4) and Bradyrhizobium sp. (NAIMCC-B-00260) were obtained from Electron Microscopy and Nanoscience Laboratory, Department of Soil Science, Punjab Agricultural University, Ludhiana and Bacterial isolate 2 (Ak-PSB-3-WHITE) was obtained from Department of Microbiology, Punjab Agricultural University, Ludhiana. Bacterial isolate 1 (CP-AK-SPS-4) and Bacterial isolate 2 (Ak-PSB-3-WHITE) were identified as Burkholderia seminalis and Burkholderia sp by sequencing of 16S rRNA gene. Further all the three cultures were evaluated for plant growth promoting features. The quantity of IAA, phosphate solubilization and gibberellic acid produced by Burkholderia seminalis was 7.05 ± 0.89 μg/ml, 137.57 ± 4.66 μg/ml and 160.73 ± 9.49 μg/ml respectively, by Burkholderia sp. was 5.37 ± 0.54 μg/ml, 130.28 ± 6.63 μg/ml and 129.94 ± 6.49 μg/ml respectively and by Bradyrhizobium sp. was 6.45 ± 0.54 μg/ml, 134.48 ± 2.78 μg/ml and 118.96 ± 3.51 μg/ml respectively. Siderophore production was exhibited only by Burkholderia seminalis (134.8 ± 4.80 μg/ml catechol type and 145.4 ± 5.09 μg/ml hydroxamate type). Liquid microbial inoculants of Burkholderia seminalis, Burkholderia sp. and Bradyrhizobium sp. were prepared using trehalose (5mM) in basal medium and showed maximum viable count upto 180 days of storage as compared to charcoal carrier based formulations at room and refrigerated temperature. The field experiment was conducted at Punjab Agricultural University, Regional Research Station, Bathinda and Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana during Kharif 2018 respectively. A total of eleven treatment combinations of liquid microbial inoculant (Burkholderia seminalis, Burkholderia sp. and Bradyrhizobium sp.) with 75% and 100% RDF were framed in randomized block design and replicated thrice. It was observed that treatment T10 (75%RDF + Burkholderia sp. + Burkholderia seminalis) significantly (p<0.05) improved vine length, stem girth, leaf length, leaf breadth, number of branches per plant, number of leaves per plant, leaf: stem ratio, green fodder yield, dry matter yield, acid detergent fiber (ADF), neutral detergent fiber (NDF), crude protein content, in vitro dry matter digestability (IVDMD), total ash content, total chlorophyll content, total sugars content and total phenolic content. Moreover, percentage increase in green fodder yield (q/ha) with T10 treatment over T1 (control) was 12.86%. Scanning electron microscopy provided insight of root colonization of forage cowpea by liquid microbial inoculants under gnotobiotic conditions, maximum root surface colonization was observed by liquid microbial inoculants of Burkholderia seminalis followed by Burkholderia sp. and Bradyrhizobium sp. which further strengthened the results of field experiments. Therefore, it can be concluded that liquid microbial inoculants could play a predominant role in integrated nutrient management of forage cowpea for enhanced productivity and quality.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810127505
dc.keywordsBradyrhizobium sp, Burkholderia seminalis, Burkholderia sp., Cowpea, Forage, Integrated nutrient management, Liquid Microbial inoculantsen_US
dc.language.isoenen_US
dc.pages123en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemIntegrated nutrient management in forage cowpea with application of liquid microbial inoculantsen_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeIntegrated nutrient management in forage cowpea with application of liquid microbial inoculantsen_US
dc.these.typeM.Scen_US
dc.titleIntegrated nutrient management in forage cowpea with application of liquid microbial inoculantsen_US
dc.typeThesisen_US
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