INTRA-UTERINE INFUSION OF HOMOLOGOUS PLASMA IN THE TREATMENT OF ENDOMETRITIS IN COWS
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Date
1995
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
The present study was undertaken on infertile cows
with uterine infections, with the objective to evaluate the
beneficial effect of intra-uterine infusion of homologous
plasma as a non antibiotic alternate therapy for bovine
endometritis. The luminal secretions of cows with clinical
endometritis allotted to group I (n=13) and II (n=10) were
aseptically collected at the time of oestrum and subjected to
plate count to assess the uterine bacterial load prior to
treatment. Cows belonging to group I were given 25 to 35 ml
of homologous plasma as intra-uterine infusion on the day of
oestrum whereas in control group (group II) the cows were
given 25 to 35 ml of sterile normal saline as intra-uterine
infusion.
Luminal secretions of all the cows were collected
aseptically at the succeeding oestrus following treatment and
the uterine bacterijal load was estimated. Cows which shov^ed
recovery in terms of clinico-gynaecological findings were
bred artificially.
Following plasma therapy definite improvement as
evidenced by clear uterine discharge, higher uterine tone and
intensity of oestrum was noticed in the treatment group.
These changes were not pronounced in the control group. The
mean uterine bacterial load showed reduction after
intra-uterine treatment in both group I and II, however the
reduction was statistically insignificant. The mean duration
from intra-uterine treatment to succeeding oestrus was
prolonged in group I (28.23 ± 4.67 d) as against normal in
group II (20.3 ± 0.4 d). The first insemination conception
rate recorded in group I was 53.85 per cent as against 40 per
cent in group II showing that there was definite improvement
in conception rate following plasma treatment.
Repeat breeder cows with subclinical first degree
endometritis allotted to group III were administered 25 to
35ml homologous plasma 24 h after insemination whereas repeat
breeders (group IV) were left untreated following
insemination. The mean conception rate for group III was
36.36 as against 20 per cent for group IV. This result also
signifies the beneficial effect of plasma treatment in
improving conception rate of repeat breeders.
An in vitro test to study the antibacterial effect of
plasma was carried out by measuring the reduction in
turbidity of a bacterial suspension on addition of plasma.
It was found that the reduction in turbidity was significant
(P<0.05) in plasma tubes compared to saline tubes. Maximum
reduction had occurred within 0 to 1 h interval in plasma
tubes. This indicated an immediate antibacterial effect of
plasma
It could be inferred from the above findings that
homologous plasma drawn from the cow at the time of oestrum
has significant bactericidal and bacteriostatic properties
and when infused into the uterus of cows affected with
endometritis helps in quicker clinical recovery and better
conception rate. Post insemination plasma infusion into the
uterus also improved conception rate in repeat breeders.
Hence plasma treatment is recommended as an effective less
expensive and non antibiotic alternate therapy for
endometritis and repeat breeding in cows.
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