EVALUATION OF MAGGOT THERAPY IN THE TREATMENT OF CHRONIC WOUND IN RAT

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dc.contributor.advisorKatoch, Rajesh
dc.contributor.authorBorkataki, Sanku
dc.date.accessioned2018-07-11T05:56:47Z
dc.date.available2018-07-11T05:56:47Z
dc.date.issued2017
dc.description.abstractThe present study was aimed to evaluate the effectiveness of maggot therapy in healing of chronic wound in non-diabetic and Streptozotocin induced diabetic Wistar rat. A total 96 male Wistar rats were separated in to two equal halves as non-diabetic and diabetic groups. Wounds were created in all and inoculated with mixed colonies of bacteria namely Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Post inoculation each group was further divided in 4 groups with 12 rats in each being represented as control, antibiotic, maggot and maggot and antibiotic treated. All treatments were applied once and held for 24 hours. Different wound kinetics in maggot treated wounds revealed significant reduction in wound area with maximum contraction, early elimination of bacterial bioburden, significant reduction of wound pH and increase in Hb level in comparison to antibiotic and control group respectively (P<0.05). Oxidative stress parameters revealed significant increase of Glutathione-S-transferase with a decreased level of Lipid peroxidation in maggot treated wounds in comparison with other groups (P<0.05). The formation of collagen tissue was also evident by higher concentration of hydroxyproline in granulation tissues in maggot treated groups. The histopathological examination of wounded tissue of maggot treated groups showed early and better epithelialization, collagenation and neovascularization with complete healing of wound in non-diabetic rat in two weeks and in diabetic rat in three weeks in comparison to antibiotic and control group, respectively. The effect of maggot and maggot with antibiotic combination, used in the present study, didn’t reveal any significant difference in healing of wound. Colonization of Lucilia sericata larvae in three different diets in room temperature showed total duration of life-cycle on red meat of sheep was shorter (12.29±0.16 days) where as in artificial diets i.e. blood agar and nutrient agar were (14.41±0.21) and (17.55±0.19) days, respectively.en_US
dc.identifier.citationNo. of references 340en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810058881
dc.keywordsChronic wound, Diabetes, Healing, Lucilia sericata, Maggot therapy, Wistar raten_US
dc.language.isoenen_US
dc.pages170en_US
dc.publisherSher-e-Kashmir University of Agricultural Sciences and Technology of Jammuen_US
dc.research.problemEVALUATION OF MAGGOT THERAPY IN THE TREATMENT OF CHRONIC WOUND IN RATen_US
dc.subVeterinary Parasitologyen_US
dc.subjectnullen_US
dc.themeEVALUATION OF MAGGOT THERAPY IN THE TREATMENT OF CHRONIC WOUND IN RATen_US
dc.these.typePh.Den_US
dc.titleEVALUATION OF MAGGOT THERAPY IN THE TREATMENT OF CHRONIC WOUND IN RATen_US
dc.typeThesisen_US
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