Phenobarbital Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks
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Date
2004
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Publisher
Anand Agricultural University, Anand
Abstract
The present work was planned to study induction of CYP3A and
CYP2H1 genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and
Quantitative RT-PCR in Bantam, Bantamized White leghorn and White leghorn
chicks. Out of 18 chicks of Bantam, Bantamized White leghorn and White leghorn, 3
from each group were treated intraperitoneal with phenobarbital at the dose rate of
12mg/100gm body weight and control group were treated with same volume of 0.9%
normal saline. After 24 hrs of medication, they were sacrificed and liver samples were
collected from each bird. Total RNA was extracted from the liver tissue samples using
Tri ReagentĀ® based method. The quantity of extracted RNA was assessed
spectrophotometrically at 260/280nm
Description
Keywords
Veterinary Pharmacology, Analysis