Effect of temperature on germination and alpha-amylase gene expression in wheat (Triticum aestivum L.)

Simple item page
dc.contributor.advisorSaini, Johar Singh
dc.contributor.authorGagandeep
dc.date.accessioned2019-09-13T04:27:30Z
dc.date.available2019-09-13T04:27:30Z
dc.date.issued2019
dc.description.abstractThe present study was conducted with the aim to determine the effect of temperature on alpha-amylase gene expression and germination in wheat. The alpha-amylase gene expression of the chromosome 2 specific gene copies was studied over three temperatures i.e. 10°C, 20°C and 30°C using real time PCR. Differential response of genotypes was observed and KSG 09, KSG220 showed maximum gene expression at 20°C, KSG 30 at 30°C and KSG 072 at 10°C correspondingly. The time period 1-2 days after imbibition was found most crucial for studying the molecular basis underlying alpha-amylase gene expression. Also, rise in gene expression was detected till day 5 and followed by decline upto day 8. Evaluation of forty genotypes for response to germination under varying temperature showed that at 29.2°C, germination percentage was significantly higher than 20.1°C and 14°C however; there was no significant difference in germination percentage between 20.1°C and 14°C. Moreover, interaction between genotype and environment was identified as key aspect for selecting heat tolerant wheat genotypes.666666666666en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810127611
dc.keywordsAlpha-amylase, Temperature, Gene expression, Germination percentageen_US
dc.language.isoenen_US
dc.pages65en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemEffect of temperature on germination and alpha-amylase gene expression in wheat (Triticum aestivum L.)en_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.themeEffect of temperature on germination and alpha-amylase gene expression in wheat (Triticum aestivum L.)en_US
dc.these.typeM.Scen_US
dc.titleEffect of temperature on germination and alpha-amylase gene expression in wheat (Triticum aestivum L.)en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
GAGANDEEP THESIS (2010).pdf
Size:
962.44 KB
Format:
Adobe Portable Document Format
Description:
M.Sc.
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis