DEVELOPMENT OF PCR BASED METHOD FOR QUANTIFICATION OF SELECTED MICROFLORA IN DAHI
| dc.contributor.advisor | Dr. J. B. Prajapati | |
| dc.contributor.author | Shovna | |
| dc.date.accessioned | 2017-03-17T09:31:56Z | |
| dc.date.available | 2017-03-17T09:31:56Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | The availability of culture-independent tools that enable a detailed investigation of the microbial biodiversity of fermented food system is the need of the hour for food microbiologists. In the present study, a real-time PCR system was standardized after designing 16S rRNA targeted genus-specific primers for quantification of selected lactic acid bacteria from dahi. We designed four primer pair specific for four predominant genera found in dahi samples i.e. Lactobacillus, Streptococcus, Lactococcus and Leuconostoc. The main objective was to standardize a multiplex assay for the enumeration of all the four genera as well as to correlate the data from traditional enumeration technique.The genotypic real time PCR technique with Real Time PCR calibration curves of pure cultures gave correlation coefficient, R2 > 0.995 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810005288 | |
| dc.keywords | MICROFLORA, DAHI, PCR BASED METHOD, QUANTIFICATION, lactic acid bacteria | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | AAU, Anand | en_US |
| dc.research.problem | DEVELOPMENT OF PCR BASED METHOD FOR QUANTIFICATION OF SELECTED MICROFLORA IN DAHI | en_US |
| dc.sub | Dairy Microbiology | en_US |
| dc.subject | dairy science, dairy microbiology | en_US |
| dc.subject | method | en_US |
| dc.theme | DEVELOPMENT OF PCR BASED METHOD | en_US |
| dc.these.type | M.Sc | |
| dc.title | DEVELOPMENT OF PCR BASED METHOD FOR QUANTIFICATION OF SELECTED MICROFLORA IN DAHI | en_US |
| dc.type | Thesis | en_US |