EVALUATION OF CULTIVATED AND WILD ORYZA SPECIES FOR IRON UPTAKE UNDER AEROBIC CONDITIONS AND VARIATION IN IRON TRANSPORTER GENES
Loading...
Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University, Ludhiana
Abstract
The cultivation of transplanted rice in northwest plains of India has lead to depletion of ground water to
almost alarming level. Replacement of rice with other water requiring crop or changed production
technology that will require less water has become essential. Cultivation of direct seeded rice under
aerobic conditions is an alternative production technology that is expected to use less water, but it will
require an altogether a different ideotype, one of the stresses the aerobic rice face is iron deficiency
induced chlorosis. The present study focused on evaluation of cultivated and wild Oryza species for iron
uptake under aerobic conditions and variation in iron transporter gene. Three hundred twenty Oryza
accessions including six Oryza glaberrima, 105 O. rufipogon, 193 O. nivara, one barthii and 15
cultivated Oryza sativa genotypes were evaluated for iron deficiency induced chlorosis (IDC), days to
50% flowering; leaf area index and SPAD value. Some genotypes were tolerant to IDC throughout the
growth period while other showed varying degree of chlorosis at the different stages. Based on the IDC
score, 59 accessions including 49 tolerant and 10 susceptible were short listed for molecular studies and
further evaluation. Basmati 370, Basmati 386, Lemont, PAU 201in cultivated varieties and O. nivara (CR
100003) and O. rufipogon (CR 100343) in wild species performed best under aerobic conditions with
minimum or no chlorosis and desirable SPAD reading and LAI. These could be useful in aerobic rice
breeding programme. In a hydroponics experiment O. rufipogon acc. CR 100001, O. nivara acc. CR
100127 and Basmati 370 showed better tolerance to IDC along with good SPAD value and high iron
content in leaves. Microsatellite markers developed from 23 iron homoeostasis gene sequence were used
for characterizing the 59 selected genotypes. Based on the variation in the 23 Fe homoeostasis genic SSRs
markers, the 59 genotypes were clustered into three groups, significance of which is discussed.
Description
Keywords
null