GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES AGAINST BACTERIAL WILT OF TOMATO CAUSED BY “Ralstonia solanacearum”

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dc.contributor.advisorPranjal Kr Kaman
dc.contributor.authorSwaroop, V. Shiva Sai
dc.date.accessioned2024-07-19T09:28:09Z
dc.date.available2024-07-19T09:28:09Z
dc.date.issued2023
dc.description.abstractRalstonia solanacearum, a major bacterial pathogen, is known to cause bacterial wilt disease in over 200 plant species (50 families) throughout the world, but is particularly devastating in the Solanaceae family. A recent survey was conducted by 458 bacterial pathologists worldwide to vote on the top ten most important pathogenic bacteria in terms of their scientific and economic importance and amongst them, R. solanacearum obtained second place in the list .The current methods of control include host crop rotation, biological soil disinfestations, and cultural practices. But these measures provide limited ability to prevent its spread .Nanotechnology is a research hot spot in modern science.this technology is capable of providing miscelaneous ways to exploit some beneficial properties of metal oxide nano particles like Anti bacterial property against pathogens and there ability to induce Nano particle Triggered systemic resistance (NTR) in plants against biotic stress by triggering the expression of defence genes in the plant cell .The aim of this work was to evaluate these two properties of nano particles. ZnO NPs were Synthesized from various Biological sources like Curcuma longa, Jatropa curcass, Pipper nigrum, Trichoderma viride, Trichoderma harzianum, Bacillus subtilis against Ralstonia solanacearum. Among which ZnO NPs from curcuma longa have shown best anti bacterial activity and the average size of curcuma longa ZnONPs was found to be 18 .5 nm and curcuma longa ZnONPs have shown 78% inhibition and 100 % inhibition at 90 and 120 ppm respectively in in vitro. Seed priming was done with both the concentrations to check the expression of PR1, PR5 and PAL genes.Final Analysis showed the Up regulated expression of all the genes under biotic stress. The Nano particle treated plants @ 90 ppm and 120 ppm have shown 22% and 30 % disease reduction respectively compared to control which showed 80% disease incidence when challenged with pathogen. These results indicate that ZnO NPs induces Systemic resistance in Tomato plants against wilt pathogen.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810212279
dc.language.isoEnglish
dc.subPlant Pathology
dc.themeGREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES AGAINST BACTERIAL WILT OF TOMATO CAUSED BY “Ralstonia solanacearum”
dc.these.typeM.Sc
dc.titleGREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES AGAINST BACTERIAL WILT OF TOMATO CAUSED BY “Ralstonia solanacearum”
dc.typeThesis
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