Inheritance studies and mapping of yellow mosaic disease resistance in an interspecific cross of mungbean (Vigna radiata (L.) Wilczek) and urdbean (Vigna mungo (L.) Hepper)
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
Yellow mosaic disease (YMD) is the most vicious viral disease causing more than 85 percent economic losses. Among Vigna species, ricebean (V. umbellata) and urdbean (V. mungo) are known to possess high level and stable resistance to YMD. In the present investigation, an interspecific cross between mungbean cv. ML 267 x urdbean cv. Mash 114 was made for inheritance studies and mapping of YMD resistance. A total of 190 were made, of which eight successfully reached pod development stage with low crossability rate of 4.21 percent. The interspecific cross (ML 267 x Mash 114) recorded lower (16.67 percent) F1 seed germination.The hybrid lethality and hybrid survivability were observed as 60 and 40 percent in F1 generation respectively. The F1 hybrid plants exhibited intermediate phenotype between the parental genotypes with respect to growth habit, leaf colour, stem colour and maturity. The true interspecific hybrids were also confirmed with molecular markers. The F2 population
derived from this cross was phenotyped for YMD resistance and segregation ratio revealed presence of two genes with duplicate gene action with chi square value of 7.35 at 0.05 level of significance. Further, a set of newly designed mungbean whole genome sequence (WGS) based SSR markers were used for validation of on different Vigna accessions. The SSR markers showed 100 percent transferability on 25 different accessions of 13 Vigna species. The obtained polymorphic information content value (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP) suggested their usefulness in different genetic
studies. Different Vigna accessions were also phenotyped for YMD resistance and V. glabrescence identified as the most promising YMD resistance source. Out of 250 WGS based SSR markers, 144 markers showed polymorphism between ML 267 and Mash 114 with 57.60 percent markers were polymorphic. These polymorphic markers along with 32 Vigna species specific markers were utilized for bulked segregant analysis (BSA) Out of 144, only one marker SSR 121 showed polymorphism between YMD resistant and susceptible bulk. Further, the SSR 121 marker showed co-segregation in F2 population with YMD resistance. The marker SSR 121 was found to be located at 6 cM distance from YMD resistance locus. The findings of study paved the way for further validation of SSR 121 and its utilization in marker assisted breeding programme in mungbean.
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Saini, Pawan (2020). Inheritance studies and mapping of yellow mosaic disease resistance in an interspecific cross of mungbean (Vigna radiata (L.) Wilczek) and urdbean (Vigna mungo (L.) Hepper) (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.