In vitro propogation in two commercial diploid bananas of Kerala
| dc.contributor.advisor | Babylatha, A P | |
| dc.contributor.author | Sapheera, C P | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2019-06-13T06:53:36Z | |
| dc.date.available | 2019-06-13T06:53:36Z | |
| dc.date.issued | 2005 | |
| dc.description | PG | en_US |
| dc.description.abstract | The studies on in vitro propagation in two commercial diploid bananas of Kerala, namely Musa (AB) ‘ Njalipoovan’ and Musa (AA) ‘ Nivedyakadali’, were carried out in the Plant Tissue Culture Laboratory, Department of Pomology and Floriculture, College of Horticulture, Vellanikkara. Sword sucker, peeper sucker and eye bud explants were used for the study. The results revealed that surface sterilization of explants with a combination treatment of absolute alcohol 70.0 per cent for 1 minute + mercuric chloride 0.1 per cent for 10 minutes + dipping in antibiotic cefotaxime 0.01 per cent solution for 15 minutes recorded cent per cent survival of explant. Physical injury of the apical dome of shoot tip explants with a longitudinal cut was found to be best in enhancing the release of axillary buds in the culture. Addition of ascorbic acid into the media at the rate of 50.0 mgl-1 reduced media and explant discolouration. For the better and faster explant establishment and growth of sword sucker and peeper sucker explants, MS medium containing BA 3.0 mgl-1 and NAA 0.5 mgl-1 was found to be best for Njalipoovan and Nivedyakadali. In shoot tip culture of Njalipoovan, maximum number of multiple shoots was recorded in the medium containing NAA 0.5 mgl-1 and BA 8.0 mgl-1 or BA 5.0 mgl-1 alone in full MS. In the case of Nivedyakadali also BA 5.0 mg l-1 in the full MS recorded the maximum number of multiple shoots. Out of the two cytokinins tried (BA and Kinetin), BA was found to be more efficient for the induction of axillary shoots in the culture. Sword sucker, peeper sucker and eye bud explants were found to be equally effective with respect to the number of axillary shoots from a single explant, in both cultivars. Sucrose 3.0 per cent and table sugar 2.0 per cent produced maximum number of multiple shoots in Njalipoovan. In the case of Nivedyakadali too table sugar at 2.0 per cent recorded maximum number of multiple shoots. Half strength of vitamins in full MS observed more number of multiple shoots in cv. Njalipoovan. In the case of cv. Nivedyakadali, half tablet of vitamin B complex (2.0 mgl-1) in the medium resulted in more number of shoots. For Njalipoovan the treatment involving full MS + NAA 2.0 mgl-1 took significantly least number of days for root initiation with cent per cent rooting. The number of roots per shoot was maximum in half MS + 10.0 mgl-1. In the case of Nivedyakadali, full MS medium containing NAA 1.0 mgl-1 took least number of days for root initiation with cent per cent rooting. Of the various carbon sources tried with Njalipoovan, number of roots produced was significantly higher in the medium containing table sugar 3.0 per cent and table sugar 2.0 per cent. In the case of Nivedyakadali number of roots produced was higher in the medium containing table sugar 3.0 per cent. Full strength of vitamins in full MS medium resulted in maximum number of roots and maximum length of roots. Nivedyakadli recorded maximum number of roots in full MS media containing half strength of vitamins whereas length of root was maximum in full strength vitamin concentration. Number of roots and length of root were higher in the media containing triademefon 1.0 mg l-1. The most suitable rooting medium for ex vitro establishment of Njalipoovan and Nivedyakadali plantlets was 1:1(v/v) mixture of vermiculite + coir pith compost. Sand + cow dung + goat manure (1: 1: 1) mixture was the best potting mixture for subsequent growth and vigour of in vitro derived diploid banana. Plantlets kept under mist chamber recorded the highest survival percentage. Mud pot, black polythene bag and white polythene bag were found to be equally effective containers for hardening of plantlets. Weekly spraying of full MS solution recorded maximum number of leaves, length of leaves, breadth of leaves and height of plantlets in both the diploid cultivars. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810108301 | |
| dc.keywords | Pomology and floriculture, Explant, Hardening off and planting out, Cost efficient methods in micropropagation, Inoculation of explants | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Department Of Pomology And Floriculture, College Of Horticulture, Vellanikkara | en_US |
| dc.sub | Horticulture | en_US |
| dc.subject | null | en_US |
| dc.theme | In vitro propogation in two commercial diploid bananas | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | In vitro propogation in two commercial diploid bananas of Kerala | en_US |
| dc.type | Thesis | en_US |