Characterization of Begomovirus(es) causing yellow mosaic disease (YMD) in mungbean
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2018
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Abstract
Begomovirus(es) causing yellow mosaic disease (YMD) on pulses during summer and kharif season in Punjab were characterized. Ninety YMD infected samples collected from different cultivated varieties, germplasm and advanced breeding lines grown at different locations were subjected to PCR amplification with two legume yellow mosaic viruses (LYMV) species specific primers. Both two species i.e. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV) were detected from all the samples. Out of ninety, DNA A and DNA B component of twenty three samples were sequenced and subjected to BLASTn. Mungbean yellow mosaic virus [Urdbean: New Delhi: 2011] accession no JQ398669.1 was prevalent in both summer and kharif season. It was observed that DNA B component was more variable than DNA A component. Alpha satellite was not found in any of the samples. Characterization of beta satellite indicated association of Cotton leaf curl Multan beta satellite with YMD infection on summer mungbean crop whereas, Malachra yellow vein mosaic beta satellite was associated with YMD infection on kharif pulses. Similar strains of virus were also observed on Amaranthus viridis, Commelina benghalensis, Cyperus rotundus, Digitaria singuanalis weed species and on hedge species Duranta. Further, out of fourteen interspecific derivative mungbean lines namely SML 1808, SML 1827, SML 1839 and SML 1840 were found resistant to YMD.
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