Phenotypic and molecular characterization of rice (Oryza sativa (L.)) genotypes for grain zinc content
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Date
2022
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Punjab Agricultural University
Abstract
Biofortification is one of the most sustainable strategies to alleviate micronutrients deficiencies for people who mainly consume rice and have limited access to diversified food. The present study aimed at evaluating a set of 160 advance breeding lines along with check varieties for various yield attributes, quality traits and grain zinc content at Ludhiana and Rauni. These were evaluated for days to 50 % flowering, plant height, ear bearing tillers per square metre, spikelets sterility, number of spikelets per panicle, thousand grain weight and grain yield, milling quality and grain zinc content. Significant genotypic variation was present for various traits at both locations. Most of the genotypes were early to medium in maturity with semi dwarf plant height and good tillering ability. Significant variation for head rice recovery was present and it ranged from (41.7 to 67.6%). The zinc content in brown rice ranged from 17.1 to 35 ppm and in polished rice from 12.3 to 29.7 ppm. The genotypes PAU 8182-205-2, PAU 8182-205-1, PAU 8182-108-1, PAU 8182-116-1, PAU 8182-150-1, PAU 8182-182-1, PAU 8182-112-5, PAU 8182-171-4, PAU 8182-175-1 and PAU 8182-215-1 had high grain zinc content of more than 24 ppm in polished rice. Grain yield was negatively correlated (-0.208** and -0.185*) with grain zinc content in brown rice and polished rice. Molecular characterization of advance breeding lines with known markers for high zinc contents did not reveal much variation as most of the primers showed monomorphic banding pattern. Zinc rich elite genotypes with desirable ancillary traits may serve as potential donors for biofortification breeding programme.
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Phyo, Su Hlaing (2022). Phenotypic and molecular characterization of rice (Oryza sativa (L.)) genotypes for grain zinc content (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.