Serosurveillance, seromonitoring and kinetics of humoral immune response against foot-and-mouth disease virus in goats
| dc.contributor.advisor | Lathar, Anshul | |
| dc.contributor.author | Kaur, Aman deep | |
| dc.date.accessioned | 2024-03-14T10:28:57Z | |
| dc.date.available | 2024-03-14T10:28:57Z | |
| dc.date.issued | 2023-07 | |
| dc.description.abstract | Small ruminants comprise the majority of the world's foot-and-mouth disease (FMD) susceptible population. But FMD surveillance and control strategies in the country largely ignore small ruminants, known to be critical in the epidemiology of the disease. Continued seromonitoring and serosurveillance of FMD in small ruminants is essential to extend support to FMD control decisions particularly regarding vaccination. In order to determine the vaccinal immune response of goats belonging to the different districts of Haryana, 416 pre-vaccination and 400 post-vaccination serum samples of goats were analyzed by SPCE. The percentage of serum samples having protective antibody titres (≥1.65 log10) during pre-vaccination was 43.2, 23.0 & 27.1 which increased to 57.2, 31.2 & 30.7 one month after FMD vaccination against FMDV serotypes O, A and Asia -1, respectively. The antibody titres were found highest against type O serotype. The E. coli expressed recombinant 3AB3 NSP based indirect ELISA were used on serum samples of goats collected from different districts of Haryana to detect the percent of infected/carrier animals in the state. Out of 903 serum samples, one hundred forty-eight (16.3%) goats were found to be positive for NSP (DIVA) reactivity providing a serological evidence of viral activity. One aspect of the work was to study the kinetics of humoral immune response in the FMDV vaccinated goats at an organized farm. In this study, forty goats (twenty kids and twenty adult) of beetle breed were vaccinated with oil adjuvanted trivalent (having FMDV serotypes O, A and Asia-1) inactivated FMD vaccine. Six goats were kept as control animals in which vaccine was not administered. Blood samples were collected regularly upto seven months. No adverse reaction was observed in the vaccinated goats. The FMDV vaccinated goats developed a strong humoral immune response against all the three FMDV serotypes with peak antibody titers observed at four weeks post-vaccination. The protective antibody levels (≥1.65 log10) persisted in both the vaccinated groups (kids as well as adults) for seven months. The mean antibody titres in adult goats were found superior and statistically significant in comparison to the kids during the study. Based on these findings, it is recommended that FMD vaccinations on a regular basis should be performed in small ruminants in order to effectively implement the FMD control plan in the state/country and achieve FMD-free status in future. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810207515 | |
| dc.language.iso | English | |
| dc.pages | 43+xiv+II | |
| dc.publisher | LUVAS Hisar | |
| dc.sub | Veterinary Microbiology | |
| dc.theme | Serosurveillance, seromonitoring and kinetics of humoral immune response against foot-and-mouth disease virus in goats | |
| dc.these.type | M.V.Sc. | |
| dc.title | Serosurveillance, seromonitoring and kinetics of humoral immune response against foot-and-mouth disease virus in goats | |
| dc.type | Thesis |