Molecular Characterization of Mungbean yellow Mosaic virus Infecting Mungbean (Vigna radiata (L.) Wilczek) in South Gujarat Agro Climatic Conditions of India
Loading...
Date
2012-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Plant Pathology Department, N. M. College of Agriculture Gujarat Agricultural University
Abstract
Mungbean or Greengram [Vigna radiala (L.) Wilczek] is
an important Pulse grown in India is a rich source of protein and
Iron. Besides nourishing the people, the cultivation of
Mungbean can sustain soils of India by adding Nitrogen through
rhizobial symbiosis. However, Yellow mosaic disease (YMD) caused
by Mungbean Yellow Mosaic Virus (MYMV) remains the most destructive
disease affecting yield potential of mungbean both quantitatively and
qualitatively. To assess the incidence of the disease in Navsari roving
field surveys were carried out during summer, 20 I I in major
mungbean growing areas of Navsari, Gandevi, Jalalpur, Vansda, Chikhli and taluka. The disease incidence ranged from
06.54 to 33.21 per cent. Crop at the first trifoliate leaf stage at NAU farm, Ugat,
and Salem villages of Navsari taluka showed 07.84-13.02 per cent incidence. The
incidence increased with the crop growth stages. Around 14.78-17.02 per cent
incidence was observed in second trifoliate leaf stage at Pepalkhed, Lakhawadi and
Vadichota villages of Vansda taluka. Disease incidence of 30.12-33.21 per cent at
Sadakpor and Pepal Gabhar villages of Ckikhli Taluka when observed just before
the flowering. The symptoms initially appeared as small scattered yellow to golden
yellow colour flecks on the infected trifoliate. The scattered symptoms gradually
enlarged covering entire leaves. Severity of the symptoms was mOre on newly
emerging leaves of the infected plant.
peR amplified and sequenced part of DNA-A molecule gave a 1285 bp nucleotide
sequence. The sequence has been submitted to GeneBank, NCBI, and was given
accession number JQQ04982. Sequence is having one gene on the virus strand and
three genes- on the complementary strand. Analysis and comparison of the
sequence with the other standard universal ORF of the DNA-A molecule indicated
that the amplified fragment have four genes viz., virus coat protein (AV I) gene
(partial cds); replication associated protein (AC3) gene (complete sequence):
transactivator protein (AC2) gene (complete cds) and replicase (AC!)
Description
Keywords
null
Citation
MUGISA CHARLES_52677