BIOCHEMICAL CHANGES INFLUENCED BY NANOPARTICLES TREATMENTS IN GERMINATING CHILLI (Capsicum annuum L.) 3008
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Date
2019-09
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JAU, JUNAGADH
Abstract
The present experiment “BIOCHEMICAL CHANGES INFLUENCED BY
NANOPARTICLES TREATMENTS IN GERMINATING CHILLI (Capsicum
annuum L.)” was conducted at Department of Biochemistry, Junagadh Agricultural
University, Junagadh with an objective to examine the different nanoparticles and
PGPR treatments respect to plant growth development stages based on morphological
and biochemical characters. In chilli, whole seedling tissue, the highest germination
percentage 70.30% was found in treatment- 6 (PGPR + Chitosan Nanoparticles). In
chilli seedling tissue, the highest root length, (2.84 cm), shoot length, (7.63 cm) and
moisture content (89.42%) was found in treatment- 6 (PGPR + Chitosan
nanoparticles). The relative water content (RWC) was found to be highest 69.98% in
treatment- 6 (PGPR + Chitosan Nanoparticles), while membrane stability index (MSI)
(67.75%) in treatment- 6 (PGPR + Chitosan Nanoparticles).
Soluble protein content in whole seedling tissues of chilli was found higher
(4.92 mg/g) in treatment- 7 (Chitosan Nanoparticles + Green silver Nanoparticles).
Also total sugar content (7.02 mg/g), free amino acid content (1.41 mg/g), total
phenol (3.39 mg/g) was found highest in treatment- 7 (Chitosan Nanoparticles +
Green silver Nanoparticles).
The highest content of chlorophyll A (0.066 mg/g), Chlorophyll B (0.019
mg/g) and total chlorophyll (0.087 mg/g) were found in treatment- 3 (PGPR- Bacillus
subtilis), while chlorophyll stability index (CSI) (1.57 mg/g) in treatment- 5 (Chitosan
Nanoparticles).Abstract
The phenol profiling of chilli variety also distinguishes difference between
different nanoparticales treatments and plant growth development stages. Total eight
phenolic acids were identified using HPLC. Higher content of Gallic acid and
salicylic acid was found in treatment-1 (control (without water)) of chilli seedling
tissues. Higher content of cinnamic acid and quercetin was found in treatment- 2
(control with water), catechol was found higher in treatment- 3 (PGPR– Bacillus
subtilis). Higher content of gallic acid and caffeic acid was found in treatment- 4
(Green silver Nanoparticles from Bacillus subtilis), vanillic acid and caffic acid was
higher in treatment- 5 (Chitosan Nanoparticles), ferulic acid and vanillic acid was
higher in treatment- 6 (PGPR + Chitosan Nanoparticles) and quercetin was found
higher in treatment- 8 (Chemically synthesized silver Nanoparticles).
The highest Superoxide dismutase (SOD) enzyme activity was found (4.67 Δ
OD·min⁻¹·gm⁻¹ Fr. Wt.) in treatment- 7 (Chitosan Nanoparticles+ Green silver
nanoparticles) and Guaiacol peroxidase (GPX) activity was highest 1290.0 Δ
OD·min⁻¹·gm⁻¹ Fr. Wt. in treatment- 6 (PGPR + Chitosan Nanoparticles). Ascorbate
peroxidase (APX) activity (44.56 Δ OD·min⁻¹·gm⁻¹ Fr. Wt.) and Polyphenol oxidase
(PPO) activity was highest (34.00 Δ OD·min⁻¹·gm⁻¹ Fr. Wt.) in treatment- 7 (Chitosan
Nanoparticles+ Green silver nanoparticles). Catalase activity was highest 43.33 Δ
OD·min⁻¹·gm⁻¹ Fr. Wt. in treatment- 6 (PGPR + Chitosan Nanoparticles). In
ascorbate peroxidise (APX) enzyme activity decreased while in super oxide dismutase
(SOD), guaiacol peroxidase (GPX) and Polyphenol oxidase (PPO) enzyme activity
was increased during plant growth development stages. Protein profiling by native
and SDS PAGE shows that all stages proteins are different. All banding pattern are
separated in different clusters. Control treatments proteins are clustered in one group
while nanoparticles treatments proteins are separated in another cluster. Among all the
treatments; nanoparticles treatments are best for morphological and biochemical
constitutes in present study.
The present investigation explained that morphological and biochemical contents
were help to distinguishes difference in different treatments during all stages of plant
growth development of chilli.