Studies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypes

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dc.contributor.advisorChawla, Neena
dc.contributor.authorInderpal Kaur
dc.date.accessioned2021-01-25T05:21:42Z
dc.date.available2021-01-25T05:21:42Z
dc.date.issued2020
dc.description.abstractThe objectives of the present study were to evaluate the changes in accumulation of antioxidants and the activities of enzymes involved in the synthesis of phenolic compounds in thirty onion genotypes during bulb development stages. The expression of gene (FLS) responsible for the synthesis of flavonol was examined in three selected genotypes, in different plant parts during the bulb development. Study included cytotoxic and apoptotic effects of selected onion extracts on cancer cell lines. Genotypic variation in phenolic content was observed in all onion genotypes during the bulb development. Total phenols, flavonols, odihydroxyphenols, anthocyanin and total antioxidant capacity was observed to be increased from onion bulb initiation stage to bulb maturation stage in most of genotypes, whereas quercetin content was highest reported during bulb developmental stage and then decreased towards maturity. Study showed that enzymes (PAL, FLS and ANS) showed increasing trend from bulb initiation to development stage followed by a decline as the bulb proceeded towards maturation. Correlation and heritability of biochemical parameters were also reported. Three onion genotypes were selected on the basis of phenolic contents and color. Selected genotypes were analysed for the expression study of FLS gene. Study showed maximum relative fold expression of FLS gene in edible portion of plant (leaves and bulb) during bulb development stage. Onion extracts from selected genotypes were prepared in two solvents (ethyl acetate and 80% methanol) to study the potential cytotoxic effects on different cancer cell lines. Combination of ethyl acetate extract of PRO-6 genotype showed significant cytotoxic effects on cancer cell lines.en_US
dc.identifier.citationInderpal Kaur (2020). Studies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypes (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810160543
dc.keywordsOnion, bulb color, development stages, qRT-PCR, Cytotoxicityen_US
dc.language.isoEnglishen_US
dc.pages134en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemStudies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypesen_US
dc.subBiochemistryen_US
dc.themeStudies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypesen_US
dc.these.typePh.Den_US
dc.titleStudies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypesen_US
dc.typeThesisen_US
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