Biochemical and molecular characterization of aerobic rice genotypes under water limiting conditions

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dc.contributor.advisorJain, Sunita
dc.contributor.authorGrewal, Savita
dc.date.accessioned2016-11-02T10:59:29Z
dc.date.available2016-11-02T10:59:29Z
dc.date.issued2011
dc.description.abstractEight indica/Basmati and aerobic rice varieties (HBC19, PS4, PAU201, HKR47, MAS25, MAS26, MAS109, and IVT3508) were used to study various physio-morphological, biochemical, agronomic and molecular parameters under water limiting conditions. For this the plants were grown in pots and were subjected to flooded as well as aerobic water conditions and in another experiment PEG induced water stress of -1 and -2 bar was generated in hydroponic conditions. Aerobic rice genotypes performed better than indica/Basmati rice varieties under aerobic and water stressed conditions in all aspects of physio-morphological, biochemical and agronomic attributes. Large deviation was observed in all rice genotypes for shoot length (30.33-47.74 cm; 38.78-41.13 cm), root length (13.73-54.33 cm; 22.40-32.58 cm), root thickness (0.47-1.38 mm; 0.42-1.19 mm), dry shoot weight (4.69-7.07 g; 0.211-255 g) and dry root weight (0.33- 1.55 g; 0.076-0.107 g), in 42-day-old seedlings when grown under aerobic conditions and in hydroponic system (at -2 bar). Relative water content (RWC) and osmotic potential reduced significantly under water limiting conditions in all the eight varieties. Aerobic rice genotypes had high basal levels of proline, free amino acids and reducing sugars as compared to the indica/basmati rice varieties under normal submerged conditions however, even though the absolute vales were still higher in aerobic varieties, the percent gain in proline, free aminoacids and reducing sugars were more in conventional varieties under aerobic and at -1 and -2 bar stress conditions in both root and shoot tissue. Yield and yield attributes were recorded at maturity only in pot experiment and here also aerobic rice varieties showed better performance than indica/Basmati rice varieties under aerobic conditions. Also the yield of aerobic rice varieties were higher under aerobic conditions (29.23-32.42 g) than under submerged conditions (21.63-28.28 g). The eight rice varieties were evaluated for molecular analysis using 10 SSR markers associated with root traits and a total of 23 alleles were detected. Notably, two SSR loci namely RM212 and RM525, had unique band of 160 bp and 110 bp size in two aerobic rice varieties i.e. MAS25 and IVT3508 respectively. An allele of 200 bp was also observed only in two aerobic rice varieties MAS25 and MAS109 at RM526 SSR locus. The average similarity of 0.5626 and 0.6523 was observed between two Basmati and two indica rice varieties to all the four aerobic rice varieties, respectively while among all the four aerobic rice varieties it was found to be only 0.4728.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/83290
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiochemistry
dc.subjectAerobic rice, Water stress, Roots, Shoots, RWC, Yield, SSR markersen_US
dc.these.typeM.Sc
dc.titleBiochemical and molecular characterization of aerobic rice genotypes under water limiting conditionsen_US
dc.typeThesisen_US
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