Standardisation of media and containers for ex-vitro establishment of anthurium plantlets produced by leaf culture
| dc.contributor.advisor | Ramachandran Nair, S | |
| dc.contributor.author | Ajith Kumar, P V | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2020-11-20T09:29:42Z | |
| dc.date.available | 2020-11-20T09:29:42Z | |
| dc.date.issued | 1993 | |
| dc.description | MSc | en_US |
| dc.description.abstract | Investigations were carried out at the Plant Tissue Culture Laboratory of the College of Agriculture, Vellayani during 1991-1993 to develop suitable methods to plant out Anthurium andreanum plantlets and to standardise media and containers to maximise the ex vitro establishment and growth of in vitro derived plantlets. Segments of leaf were used as explant for reproducing required number of plantlets for the study. Various factors influencing in vitro rooting were standardised. Plantlets of 3 cm length with at least three leaves recorded shortest time (10.24 days) for root initiation and produced maximum number of roots per shoot compared to smaller shoots. Combination of BA 0.5 ppm and IAA 2.0 ppm was found to be the best for in vitro rooting. Agar at 0.7 % recorded shortest time (10.54 days) for root initiation and the number of roots per shoots decreased by increasing its concentration in the medium, while the length 0f ro0t increased along with increase in agar concentration. Sucrose level maintained at normal level in MS medium (3.0 per cent) was found to be the best for in vitro rooting. In order to standardise the media and containers for ex vitro establishment, media such as coarse sand, finesand, charcoal, soilrite and sphagnum moss and containers such as mud pot, plastic pot, paper pot, polythene cover and netted pot were used. Plantlets with at least 2.5-3 cm size (with 3-4 leaves and two or more roots) recorded 90.0 to 100.0 per cent survival irrespective of media and containers. Of the various madia and containers tried plastic pot as the container and soilrite as the media recorded highest number of leaves in the transplanted plants at fortnightly intervals. Both one and two months after transplanting, mud pot outdid other containers and soilrite outdid other media with respect to plant height. In the case of leaf area at second and fourth fortnight, plastic pot and at third fortnight polythene cover was found to be the best container, and soilrite was the best medium at third fortnight onwards. The containers showed no significant influence on petiole length at second and fourth fortnight but at second fortnight onwards medium soilrite recorded maximum petiole length. The plants grown in polythene cover with media soilrite recorded inaximum number of roots and length of roots at two months after transplanting. So it is evident that among the media, soilrite was the best for ex vitro establishment of anthurium plantlets but containers showed no uniform response with various growth factors. | en_US |
| dc.identifier.citation | 170973 | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810155384 | |
| dc.keywords | Horticulture | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Department of Horticulture, College of Agriculture, Vellayani | en_US |
| dc.sub | Horticulture | en_US |
| dc.theme | Media and containers for ex-vitro establishment of anthurium plantlets produced by leaf culture | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Standardisation of media and containers for ex-vitro establishment of anthurium plantlets produced by leaf culture | en_US |
| dc.type | Thesis | en_US |