Effect of Imidacloprid- A Neonicotinoid Insecticide on Reproductive Functions of Female Rats

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dc.contributor.advisorPunia, J.S.
dc.contributor.authorLohiya, Archana
dc.date.accessioned2017-07-28T04:28:00Z
dc.date.available2017-07-28T04:28:00Z
dc.date.issued2013
dc.description.abstractImidacloprid, a neonicotinoid insecticide is widely and most commonly used insecticide in soil, seed and foliar application for the control of suckling insects including rice hoppers, aphids, whiteflies, termites, turf insects, soil insects and some beetles and as an ectoparasiticide in veterinary medicine, Yet its effects on reproductive system of nontarget mammals have not been studied extensively. Study to assess the effects of imidacloprid on reproductive system was carried out in female wistar rats at two dose levels (19 and 38 mg/kg/day) administered for 10, 20 and 30 days. Effects were compared with respective control animals administerd daily with 2% gum acasia (1ml/100g b.wt.) for 10, 20 and 30 days. Different parameters undertaken were body weight, body organ weight, oxidative stress parameters viz. antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx)), reduced glutathione (GSH), and the lipid peroxidation (LPO) product malondialdehyde (MDA), cytoplasmic and membrane proteins in different organs (liver, kidney, ovary, uterus) and blood, microelements (zinc, copper and iron) level in ovary, progesterone level in plasma, and histopathological changes of vital organs. Imidacloprid at 38 mg/kg dose administered daily for 10, 20 and 30 days significantly increased levels of MDA and relative organ weights of liver and kidney after 20 and 30 days post administration. In ovary and plasma increased levels of MDA was observed at 38 mg/kg dose administered for 30 days. There was significant decrease in level of SOD enzyme in liver, ovary, uterus and in red blood cells at 38 mg/kg dose of imidacloprid administered daily for 20 and 30 days; GSH level was decreased in red blood cells; GPx enzyme levels were decreased in liver and ovary; and cytoplasmic and membrane proteins were decreased in liver and kidney tissues. At 38 mg/kg dose of imidacloprid administered daily for 30 days there was significant decrease in SOD enzyme level in kidney; GSH levels in both liver and kidney, GPx enzyme level in kidney and uterus. Cytoplasmic and membrane proteins were decreased in kidney, uterus, ovary and in red blood cells. No significant change in level of copper and iron was observed at both the doses. Zinc level was significantly increased in ovaries and serum progesterone level was decreased at 38 mg/kg dose administered daily for 30 days. Histopathological changes were observed in liver, kidney and ovary at 38 mg/kg dose administered daily for 20 and 30 days. Histopathological changes in uterus were observed at 38 mg/kg dose administered daily for 30 days. No significant changes were observed at 19 mg/kg dose of imidacloprid administered daily for 10, 20 and 30 days.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810026425
dc.keywordsImidacloprid, Progesterone, malondialdehyde (MDA), glutathione peroxidase (GPx), reduced glutathione (GSH), superoxide dismutase (SOD), cytoplasmic and membrane proteins, copper, iron, zinc, histopathology.en_US
dc.language.isoenen_US
dc.publisherLUVASen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.themeEffect of Imidacloprid- A Neonicotinoid Insecticide on Reproductive Functions of Female Ratsen_US
dc.these.typeM.V.Sc.en_US
dc.titleEffect of Imidacloprid- A Neonicotinoid Insecticide on Reproductive Functions of Female Ratsen_US
dc.typeThesisen_US
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