Enhancing productivity and industrial sustainability of snack seeded pumpkin through interspecific hybridization
dc.contributor.author | Garcha, Karmvir Singh | |
dc.date.accessioned | 2023-06-28T14:20:42Z | |
dc.date.available | 2023-06-28T14:20:42Z | |
dc.date.issued | 2022 | |
dc.description.abstract | seeded pumpkin through interspecific hybridization’ was planned to combine hull-less seed, bush growth habit and virus resistance in a single Cucurbita genotype. Interspecific hybridizations among ten C. pepo (hull-less) and two C. moschata (hulled) rendered HLP-36 (C. pepo) and PVR1343 (C. moschata) as the best cross compatible genotypes. Molecular characterization of the virus symptoms revealed the predominance of two begomovirus species viz., Squash Leaf Curl China Virus and Tomato Leaf Curl New Delhi Virus. The intraspecific F2 populations of C. moschata displayed monogenic dominant and digenic recessive inheritance for bush growth habit and begomovirus resistance, respectively, whereas for hull-less seed monogenic recessive expression was observed in C. pepo. However, these best fit ratios were not found with the interspecific F2 populations due to genomic instability among C. pepo and C. moschata. Multiple crosses involving HLP-36, HLP-639 and PVR-1343 led to development of three genotypes having all the targeted traits. To map hull-less character, a 2.06 Mb genomic region on chromosome 12 of C. pepo was used for developing 21 KASP assays. Genotyping of F2 population derived from HP111 × HLP36 cross with eight polymorphic KASP assays lead to limit the hull-less seed locus (Cphl-1) to 40.3cM partial genetic linkage map. The most closely linked marker viz., Cp_3430407 (10 cM) accurately predicted 93.33% hull-less genotypes in the diversity panel. The functional analysis of candidate genomic region decoded 182 genes, out of which 19 shared homology with A. thaliana genes related to cell wall, cellulose or lignin biosynthesis. Two of these genes viz., ‘NAC’ and ‘MYB’ act as master regulators of lignin biosynthesis and were differentially expressed in hulled and hull-less seeds at different time intervals. The transcriptional differences between hull-less and hulled seeds at 15 and 20 days after pollinations displayed 3124 differentially expressed genes. Out of these 482 (15.43%) were related to cell wall biosynthesis, which indicated the seed coat initiation between 15 to 20 DAP. Further, functional characterization of the gene(s) involved in the lignin/seed coat development will improve the understanding of hull development in pumpkin. The newly developed hull-less lines with bush growth habit and begomovirus resistance will revolutionize the hull-less seed industry, while the KASP markers will be highly useful in marker assisted selections from the segregating populations. | en_US |
dc.identifier.citation | Garcha, Karmvir Singh (2022). Enhancing productivity and industrial sustainability of snack seeded pumpkin through interspecific hybridization (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810197820 | |
dc.keywords | C. pepo, C. moschata, Interspecific hybridization, KASP, Linkage map | en_US |
dc.language.iso | English | en_US |
dc.pages | 114 | en_US |
dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
dc.research.problem | Enhancing productivity and industrial sustainability of snack seeded pumpkin through interspecific hybridization | en_US |
dc.sub | Vegetable Science | en_US |
dc.theme | Enhancing productivity and industrial sustainability of snack seeded pumpkin through interspecific hybridization | en_US |
dc.these.type | Ph.D | en_US |
dc.title | Enhancing productivity and industrial sustainability of snack seeded pumpkin through interspecific hybridization | en_US |
dc.type | Thesis | en_US |