Effect of glycine betaine and proline on seed germination and seedling growth of oat (Avena sativa L.) under salt stress
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
In present investigation, seeds of oats (Avena sativa L.) were subjected to different
concentrations of salt stress (25mM, 50mM, 75mM 100mM) in OL-1896 and OL-1962
genotypes to study the effects of salinity on physiological (seed germination, seedling length,
biomass, seed vigour and speed of germination) and biochemical parameters (total soluble
sugars, proteins, MDA and activities of different antioxidant enzymes viz SOD, POX and
CAT). Physiological parameters decreased with increasing salinity level whereas out of
biochemical parameters; total soluble sugars, SOD, POX and MDA activity increased and
total soluble proteins and CAT decreased. From the above stress levels used, two stress levels
i.e. 50mM and 75mM were selected and seeds were treated with different concentrations of
proline (5mM and 10mM) and glycine betaine (10mM and 20mM) to study their influence on
physiological as well as biological parameters under salt stress conditions. The results showed
that the seeds treatments (PRO 5mM, 10mM and GB 10mM, 20mM) improved the activity of
physiological and biochemical parameters and reduced the harmful effects of salt stress in
both genotypes of oats. Genotype OL-1896 was comparatively more tolerant to salt stress.
Also, proline 10mM and glycine betaine 20mM was found to be more effective than proline
5mM and glycine betaine 10mM. Thus, the results suggest that exogenous application of
proline and glycine betaine increased salt tolerance in oat seedlings.
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Makkar, Astha (2020). Effect of glycine betaine and proline on seed germination and seedling growth of oat (Avena sativa L.) under salt stress (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.