CHARACTERISATION AND ANTIBIOGRAM PROFILE OF ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS ISOLATED FROM BOVINE MASTITIC MILK
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Date
2015
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
Mastitis is one of the economically important disease throughout the
world. Subclinical mastitis is characterized by no structural changes in udder or
consistency of milk is a significant public health hazard as the mastitic milk enters
the human food chain as normal milk (Hameed et al., 2006). Heat stable
enterotoxins produced by the pathogenic bacteria, antimicrobial resistance and
antibiotic residues which are associated with mastitis makes it public health
significance day by day. The study was undertaken for a period of 10 months
from June 2014 to March 2015 during which a total of 100 mastitic milk samples
both from clinical and sub-clinical mastitic cases identified by California Mastitis
Test (CMT) and changes in the consistency of milk and udder were collected.
Epidemiological investigation of the zoonotic bacteria present in mastitic milk
was carried out for a total of 100 samples each of dung and udder wash and 50
each of milker’s hand wash, fodder, water and soil samples were collected from
three different regions namely, Thariyode and Pozhuthana panchayaths and
Kalpetta Municipality. A total of 62 mastitic samples were found positive for S.
aureus of which 21 were obtained from Kalpetta, 23 from Thariyode and 18 from
Pozhuthana. Seven S. aureus isolates were found positive for MRSA. E. coli was
isolated from 51 mastitic milk samples which included 24 from Kalpetta, 20 from
Thariyode and seven from Pozhuthana. L. monocytogenes was detected in six
mastitic milk samples with three, two and one from Kalpetta, Thariyode and
Pozhuthana respectively. Salmonella spp. was isolated from three mastitic milk
samples, two from Kalpetta and one from Pozhuthana. The antibiotic sensitivity
revealed that isolates were found highly sensitive to ciprofloxacin, ceftriaxone and
chloramphenicol, moderately sensitive to gentamicin, streptomycin and
tetracyclin but found resistant to methicillin, ampicillin, amoxyclav and
vancomycin.
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