MOLECULAR DETECTION AND CHARACTERISATION OF INFECTIOUS BURSAL DISEASE VIRUS (IBDV) IN POULTRY
| dc.contributor.advisor | R., Rajasekhar | |
| dc.contributor.author | D., NANDHAKUMAR | |
| dc.date.accessioned | 2021-01-12T10:01:20Z | |
| dc.date.available | 2021-01-12T10:01:20Z | |
| dc.date.issued | 2019-07-15 | |
| dc.description.abstract | Recurrent infectious bursal disease (IBD) outbreaks were reported in different regions of Kerala, India. This study reports the molecular detection and comparative genetic analysis of the hypervariable region of the VP2 gene of IBD virus from the field outbreaks in Kerala. Genetic analysis of 22 isolates from 41 suspected field outbreaks of IBD were carried out in this study. In phylogenetic analysis, the obtained field isolates fall into genogroup 1 and 3. In genogroup 3, all vvIBDV isolates shared a common ancestor with other south Indian isolates but isolates 9/CVASP/IBDV, 10/CVASP/IBDV, 12/CVASP/IBDV, 14/CVASP/IBDV and 17/CVASP/IBDV are most recently evolved and is diverged from the south Indian isolates and other isolates obtained in the study. The amino acid sequence of 22 isolates were analysed, out of which 18 had serine rich heptapeptide virulence sequence ‘SWSASGS’ and conserved amino acids which are characteristic of vvIBDV. In all the vvIBDV isolates obtained in the study had phenylalanine and Valine at the position 240 and 294 respectively similar to recently evolved Indian IBDV isolates. But we observed T269A and S299N mutations in the isolate 6/CVASP/IBDV and is the first report of such mutations at this positions in India IBDV isolates. The isolate 11/CVASP/IBDV had a unique mutation of V225A which is not yet reported in IBDV isolates. Among these 18 isolates, 9 isolates obtained from vaccinated flocks. Two isolates (15/CVASP/IBDV and 18/CVASP/IBDV) were similar to intermediate plus vaccine strain. The isolates 8/CVASP/IBDV and 19/CVASP/IBDV had amino acids unique for the intermediate vaccine with mutations observed at H253Q and V256I in 19/CVASP/IBDV, T270A and novel mutation N279Y in isolate 8/CVASP/IBDV. These two isolates had non-virulent classical heptapeptide sequence ‘SWSARGS’ nevertheless they produce field outbreaks of IBD. This is the first genetic characterisation study of field IBDV isolates in Kerala, India | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810159935 | |
| dc.keywords | VP1 gene,VP2 gene,VP3 gene.VP4 gene,VP5 gene,Reverse Transcriptase Polymerase Chain Reaction (RT-PCR),IBD-immune complex vaccines | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 105 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD | en_US |
| dc.research.problem | INFECTIOUS BURSAL DISEASE VIRUS (IBDV) IN POULTRY | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.theme | MOLECULAR DETECTION AND CHARACTERISATION OF INFECTIOUS BURSAL DISEASE VIRUS (IBDV) IN POULTRY | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | MOLECULAR DETECTION AND CHARACTERISATION OF INFECTIOUS BURSAL DISEASE VIRUS (IBDV) IN POULTRY | en_US |
| dc.type | Thesis | en_US |