Isolation, characterization and molecular diversity of endophytic bacteria from the root nodules of aea (Pisum sativum L.)
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Date
2011
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CCSHAU
Abstract
Endophytes have a symbiotic relationship with the different parts of plants and could play a
very important role in supporting the plant growth. Endophytic bacteria of different genera exhibit
various type of beneficial characters and are known to promote plant growth. Therefore, in the present
investigation, a total of 60 nodule endophytic bacteria were isolated from the surface sterilized root
nodules of pea. Most of the pea nodule endophytic bacterial isolates were gram positive and almost all
the gram positive isolates were spore formers. All the pea nodule endophytic bacteria were screened for
the presence of beneficial characters, and found that 63.3% of endophytic isolates were promoting root
growth of pea seedlings and 38.3% of pea nodule endophytic bacteria were phosphate solubilizers,
83.3% of pea nodule endophytes were ammonia producers and only 32% of isolates were producing
organic acid. Molecular diversity of selected 48 endophytic isolates showed that there was wide
diversity among pea nodule bacterial endophytes and atleast 17 biotypes were present in pea nodules at
80% level of similarity coefficient. Biotype I was present in 33% of nodules and biotype II was present
in 19% of the pea nodules. Selected 41 isolates were screened under pot culture conditions using pea as
test host. Inoculation of pea nodule endophytes promoted the growth parameters of pea and enhanced
nitrogen fixation, the most efficient isolates were PNE15, PNE17 and PNE26.
Description
Keywords
Root nodulation, Bacteria, Planting, Peas, Biological development, Vegetative propagation, Growth promoters, Acidity, Productivity, Application methods