Protective role of spermine on salinity induced oxidative stress in rice (Oryza sativa L.) seedlings

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dc.contributor.advisorMalhotra, Sarla
dc.contributor.authorSnehvart
dc.date.accessioned2016-11-15T11:35:05Z
dc.date.available2016-11-15T11:35:05Z
dc.date.issued2010
dc.description.abstractThe effect of salt stress and spermine was studied on oxidative stress and antioxidative system in rice seedlings. The seeds of salt-tolerant (CSR-10) and salt-sensitive (IR-28) cultivars of rice were germinated on Whatman filter paper No. 1 for 4 days and then the seedlings were transferred to hydroponic culture system. The three week old rice seedlings were subjected to 50 and 100 mM salt stress for 10 days. The protective effect of spermine was studied by its exogenous application at 0.1 and 1 mM concentration before salt stress or along with salt stress or after salt stress. The roots and leaves of control, salt-stressed and spermine treated stress plants were analysed for lipid peroxidation (MDA, O2.-, H2O2 content and LOX activity), activities of antioxidative enzymes (CAT, SOD, GR, POX and APX) and antioxidative metabolites (ascorbate and glutathione). Imposition of stress resulted in increase in H2O2, O2.- and MDA content and LOX activity, however, the increase was more pronounced in IR-28. The activities of antioxidative enzymes, in general, decreased in both the tissues of IR-28 but increased in those of CSR-10 upon exposure to salinity. Exogenous application of spermine could partially alleviate the deleterious effect of salinity by lowering H2O2, O2.-, MDA content and LOX activity both in leaves and roots of salt stressed plants of both cultivars. The level of ascorbic acid and reduced glutathione content increased by spermine application to stressed plants of both cultivars. All antioxidative enzymes exhibited an increase in activities in both cultivars after spermine application but the higher concentration (1 mM) had no effect on GR and SOD activity in leaves of CSR-10. Thus, PAs are able to influence oxidative stress intensity by moderating the activities of ROS scavenging enzymes.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/85567
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiochemistry
dc.subjectEnzymes, Food preservation, Planting, Amines, Tolerance, Vegetative propagation, Antioxidants, Inorganic compounds, Rice, Fruitsen_US
dc.these.typeM.Sc
dc.titleProtective role of spermine on salinity induced oxidative stress in rice (Oryza sativa L.) seedlingsen_US
dc.typeThesisen_US
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