Morpho-physiological and molecular characterization of wheat-rye lines carrying 5R chromosome and its transfer into wheat background for nutrient use efficiency

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dc.contributor.advisorSohu, V.S.
dc.contributor.authorAyoubi, Habiburahman
dc.date.accessioned2023-06-25T07:49:11Z
dc.date.available2023-06-25T07:49:11Z
dc.date.issued2022
dc.description.abstractThe aim of present study is to understand and confirm the role of 5R chromosome in micronutrient deficiency tolerance in triticale/wheat derivatives and to explore the genes related to micronutrient deficiency tolerance, transfer of 5R into wheat backgrounds known for NUE and the interaction of micronutrient uptake efficiency with nitrogen uptake. Therefore, in this investigation we evaluated a set of 316 lines derived from TRITICALE X WHEAT crosses and these were evaluated for field traits for characterization. The traits like plant height, presence of awns, number of grains, grain weight etc was observed. A total of 32 molecular markers specific to a particular arm of rye chromosome were used. The result showed that different lines carried different arms of a particular rye chromosome. The number of lines found for 1RS (54 lines) and 1RL (76 lines) to carry to short and long arms of rye chromosomes respectively. For the second chromosome of rye, 2RS and 2RL (34 lines) were carried for 2RS and (93 lines) were identified for 2RL, respectively. The number of lines identified for the rye chromosome of 3RS was (131 lines) and (51 lines) were carried out for 3RL respectively. The number of lines observed for the rye chromosome of 4RS was (36 lines) to carry the short arm of the fourth chromosome of rye and (124 lines) were found to carry the long arm of the fourth chromosome. For the short arm of the fifth chromosome of rye we identified (27 lines) while we found (82 lines) for the long arm of the fifth chromosome of rye respectively. The number of lines found for the rye chromosome of 6RS was (84 lines) to carry the short arm of the sixth chromosome of rye and (76 lines) were found to carry the long arm of the sixth chromosome of rye. Finally the number of lines found for the rye chromosome of 7RS was (46 lines) to carry the short arm of seventh chromosome of rye and (24 lines) were identified for 7RL respectively. 5R lines are found to be good in phytosiderophore content. The mean PSs of 15 5R including checks was in barely followed by rye, MT-8-SNO.38 and MT-8-SNO.45. Further the 5R chromosome was transferred to wheat background PBW677 and the F2 generation plants positive for 5R from the same was subjected to estimation of PS content. We estimated 28 5R positive and 11, 5R negative plants. In positive lines the mean of plant number 14 was found to be highest and the lowest mean vale for plant number 22.en_US
dc.identifier.citationAyoubi, Habiburahman (2022). Morpho-physiological and molecular characterization of wheat-rye lines carrying 5R chromosome and its transfer into wheat background for nutrient use efficiency (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810197724
dc.keywordsRye chromosomes, triticale, phytosiderophore, PBW867en_US
dc.language.isoEnglishen_US
dc.pages82en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemMorpho-physiological and molecular characterization of wheat-rye lines carrying 5R chromosome and its transfer into wheat background for nutrient use efficiencyen_US
dc.subGenetics and Plant Breedingen_US
dc.themeMorpho-physiological and molecular characterization of wheat-rye lines carrying 5R chromosome and its transfer into wheat background for nutrient use efficiencyen_US
dc.these.typePh.Den_US
dc.titleMorpho-physiological and molecular characterization of wheat-rye lines carrying 5R chromosome and its transfer into wheat background for nutrient use efficiencyen_US
dc.typeThesisen_US
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