Evaluation of oxidative stress and genotoxicity in carps of south-western region of Punjab
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The carps i.e. Catla (Catla catla) and Mrigal (Cirrhinus mrigala) were collected from three
different locations i.e. Ludhiana, Muktsar and Bathinda. The content of proteins, activity of
antioxidant enzymes (SOD, CAT, GPx,GR), level of LPO and GSH in organs (liver, kidney,
gills) and muscles of carps, heavy metal content, genotoxicity and qualitative analysis of
proteins in liver of carps was estimated. The protein content, activity of GR and level of GSH
was found to be present in order: Ludhiana > Bathinda >Muktsar whereas, activities of
antioxidant enzymes(SOD, CAT, GPx) and levels of LPO were in the order: Muktsar
>Bathinda > Ludhiana in all the organs and muscles of both the carps. Heavy metals (As and
Cr) were found in the liver of both carps of district Muktsar and Bathinda above the
permissible limits. DNA damage determined by genotoxicity (Comet assay) was also found to
be high in the liver of carps of district Muktsar followed by Bathinda and Ludhiana.
Qualitative analysis of proteins (SDS-PAGE) revealed protein bands (32 KDa, 38KDa) were
present in liver of Catla and Mrigal of both Muktsar and Bathinda whereas some of the
protein bands (120 KDa, 150 KDa and 200 KDa) were observed only in Mrigal of Bathinda.
The carps of South-western region of Punjab (Muktsar and Bathinda) are more prone to
oxidative stress due to groundwater contaminants.
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Citation
Money (2021). Evaluation of oxidative stress and genotoxicity in carps of south-western region of Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.