“Evaluation of Gender Pre-selection through Sperm Enrichment Techniques Using Real-Time PCR in Bovines
Loading...
Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Anand Agricultural University, Anand
Abstract
The present work was undertaken to assess the efficiency of the classical X and Y sperm enrichment methods, viz., sperm swim-up, gravity sedimentation, Sephadex filtration, and discontinuous Percoll density gradient centrifugation, using the modern molecular technique of Real-Time PCR or qPCR in buffalo and cow bulls, in the Department of Animal Biotechnology and Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & AH, Anand Agricultural University, Anand.
Total of 12 semen ejaculates six each from buffalo and cow bull, diluted
Description
Keywords
Animal Biotechnology, Analysis