Influence of epibrassinolide on physiobiochemical traits of wheat (Triticum aestivum L.) under water deficit at vegetative stage
dc.contributor.advisor | Pradhan, Jyostnarani | |
dc.contributor.author | Sharma, Amit Kumar | |
dc.date.accessioned | 2023-01-19T10:48:50Z | |
dc.date.available | 2023-01-19T10:48:50Z | |
dc.date.issued | 2022 | |
dc.description.abstract | Water deficit have been observed frequently, especially in rain-fed wheat, causing severe yield losses in most wheat grown regions of the world. Its impact on crop productivity on growth differentiation development, dry matter accumulation, and productivity might vary substantially depending on the genetic composition of the plant. A class of phytohormones known as brassinolide is crucial for plant development and adoptability to environmental stresses. This study sough to identified changes in physio-biochemical processes due to exogenous application of brassinolide regarded the alleviation of negative impact of low moisture stress in wheat crop. This experiment was conducted with three objectives (1) To screen out the genotypes for water deficit stress and to identify contrasting sets on the basis of physiological traits. (2) To compare the morpho-physiological traits under water deficit during the vegetative stage of wheat. (3) To investigate the influence of epibrassinolide in relation to water deficit during vegetative stage of wheat. For the first objective, 10 cultivars were taken under four concentrations of PEG 6000 (5%, 7.5%, 10%, 15%) along with control (distilled water) in petri plates. For identification of contrasting genotypes, seedling parameters viz. emergence percentage, biomass of seedling, seedling length, seedling vigour index I and seedling vigour index II were recorded in 10 days old wheat seedlings. On the basis of growth performances one set of contrasting wheat genotypes were identified (HD-2733, relatively tolerant for stress and DBW-187 relatively stress sensitive). Similarly, BRs concentration was chosen by taking EBL in 4 concentrations along with control and 0.01mM EBL stood best among all. Taking all these results in to consideration, second experiment was performed, where two genotypes along with four treatments (well-watered, water deficit, EBL treated and EBL + water deficit) were maintained for other two objectives. Different physio-biochemical parameters were compared across the treatments and genotypes. The results are indicating that the tolerant genotype (HD-2733) showed better tolerance across all most all morphophysiological and biochemical parameters as of sensitive genotypes (DBW-187). It was clear that low moisture stress had a detrimental impact on the growth, development, physiology, and yield of wheat plant, and that a lower dose of EBL was effective against water deficit condition. Under conditions of water scarcity, EBL increase the relative water content, membrane stability index, root length, chlorophyll content, and carbohydrate and protein content. Furthermore, the application of EBL positively balanced the osmolyte such as proline, controlled the amount of phenol and malondialdehyde (MDA) content under stress condition. The growth response of HD- 2733 was higher than that of DBW-187 when exposed to EBL under low moisture, over all the EBL play a major role in enhancement of growth biomass, yield and decrease membrane damage in wheat under water deficit. However further investigations with EBL are needed to understand the mechanism of its action. | en_US |
dc.identifier.other | M /BPP/422/2020-21 | |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810192587 | |
dc.keywords | Epibrassinolide, physiobiochemical, traits, wheat, Triticum aestivum L, water deficit, vegetative stage, Plant Physiology. | en_US |
dc.language.iso | English | en_US |
dc.pages | 103, i-xx (Bibliography) | en_US |
dc.publisher | DRPCAU, PUSA | en_US |
dc.sub | Plant Physiology | en_US |
dc.theme | Influence of epibrassinolide on physiobiochemical traits of wheat (Triticum aestivum L.) under water deficit at vegetative stage | en_US |
dc.these.type | M.Sc | en_US |
dc.title | Influence of epibrassinolide on physiobiochemical traits of wheat (Triticum aestivum L.) under water deficit at vegetative stage | en_US |
dc.type | Thesis | en_US |