Fine mapping of Bacterial Blight resistant gene from Oryza glaberrima Steud. and expression analysis of putative candidate genes in the region

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dc.contributor.advisorKumari Neelam
dc.contributor.authorBabbar, Ankita
dc.date.accessioned2021-10-27T09:22:30Z
dc.date.available2021-10-27T09:22:30Z
dc.date.issued2021
dc.description.abstractBacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating disease of rice leading to huge yield losses in Southeast Asia. A bacterial blight recessive gene xa-45(t) was identified from the O. glaberrima accession IRGC102600B and a QTL was mapped to the rice chromosome 8. This QTL limits an 80 kb region on Nipponbare reference genome IRGSP-1.0 harboring 9 candidate genes. Fine mapping of this region was carried out using a RIL population developed from the cross of rice cultivar Pusa 44 and an Introgression line IL274. The F6 and F7 RIL population were phenotypically evaluated by leaf clipping method against Xoo pathotype-VII. The phenotypic assessment confirms 1:1 segregation ratio of the gene in the mapping population indicating single gene control of BB resistance by xa-45(t). For narrowing down the 80 kb region, the sequencing of all the nine candidate genes were performed using the two parents. The presence of 18 SNPs and Indels were observed between the parents of which 7 were used for the marker’s development. The analysis of the genotypic data confirms that among the 9 loci, the LOC_Os08g42410 could be considered as the putative candidate for the bacterial blight resistance gene xa-45(t). Further, relative expression analysis of the candidate genes at different time intervals of BB infection showed over expression of LOC_Os08g42410 specific transcripts and thus strongly supports its candidacy towards the bacterial blight gene xa-45(t).en_US
dc.identifier.citationBabbar, Ankita (2021). Fine mapping of Bacterial Blight resistant gene from Oryza glaberrima Steud. and expression analysis of putative candidate genes in the region (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810177423
dc.keywordsBacterial blight, Oryza glaberrima, Bacterial blight recessive genes, Quantitative trait loci, Single Nucleotide polymorphism and Insertion-Deletions. (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.language.isoEnglishen_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemFine mapping of Bacterial Blight resistant gene from Oryza glaberrima Steud. and expression analysis of putative candidate genes in the regionen_US
dc.subAgricultural Biotechnologyen_US
dc.themeFine mapping of Bacterial Blight resistant gene from Oryza glaberrima Steud. and expression analysis of putative candidate genes in the regionen_US
dc.these.typeM.Scen_US
dc.titleFine mapping of Bacterial Blight resistant gene from Oryza glaberrima Steud. and expression analysis of putative candidate genes in the regionen_US
dc.typeThesisen_US
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