IMMUNODIAGNOSIS OF BOVINE GASTROTHYLACOSIS USING COPROANTIGENS

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dc.contributor.advisorK. Devada
dc.contributor.authorA KANDASAMY
dc.date.accessioned2020-03-12T07:41:43Z
dc.date.available2020-03-12T07:41:43Z
dc.date.issued2000
dc.description.abstractA study was conducted on the prevalence of paramphistomatidosis in Thrissur from June 1999 to May 2000. feasibility of coproantigen detection by ELISA and comparison of sensitivity of ELISA using coproantigens and ELISA using serum antibodies in diagnosis of gastrothylacosis, caused by Gastrothylax crumenifer, in cattle. It was noted from the registers maintained at the University Veterinary Hospitals at Kokkalai and Mannuthy and that at the Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, that out of a total number of 1534 faecal samples from bovines examined, 253 (16.5 per cent) animals were found to be positive for amphistome eggs with the maximum prevalence (23 per cent) in June and July. Generally the infection was prevalent throughout the year. An indirect ELISA using rabbit hyperimmune serum against somatic antigens of G. crumenifer was performed to detect coproantigens in faecal samples collected from 100 known G. crumenifer infected cattle. Seventy four samples were found to contain detectable levels of coproantigen indicating a sensitivity of 74 per cent. Serum samples collected from the same infected cattle were tested for antibodies to G. crumenifer by an indirect ELISA using somatic antigens. Fifty one samples were found positive for antibodies indicating a sensitivity of 51 percent. It was seen that when 43 cattle were positive for both coproantigens and serum antibodies, 18 cattle were negative for both of them. Although 31 cattle which were negative for serum antibodies were found positive for coproantigens, eight cattle negative for coproantigens were found positive for serum antibodies. The results showed that coproantigen detection, which revealed a higher sensitivity than the detection of serum antibodies by ELISA, is feasible for the diagnosis of gastrothylacosis in bovines.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145255
dc.keywordsIMMUNODIAGNOSIS OF BOVINE GASTROTHYLACOSIS USING COPROANTIGENSen_US
dc.language.isoenen_US
dc.pages62en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemIMMUNODIAGNOSIS OF BOVINE GASTROTHYLACOSIS USING COPROANTIGENSen_US
dc.subVeterinary Parasitologyen_US
dc.subjectnullen_US
dc.themeIMMUNODIAGNOSIS OF BOVINE GASTROTHYLACOSIS USING COPROANTIGENSen_US
dc.these.typeM.V.Sc.en_US
dc.titleIMMUNODIAGNOSIS OF BOVINE GASTROTHYLACOSIS USING COPROANTIGENSen_US
dc.typeThesisen_US
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